Title:
A fluorescence euphotic device and the fluorescence euphotic method
Document Type and Number:
Japanese Patent JP6276749
Kind Code:
B2
Abstract:
A fluorescence receiving apparatus comprises an excitation light source, a spatial light modulator of a phase modulation type for phase-modulating excitation light to obtain phase-modulated light, a focusing optical system configured to focus the phase-modulated light to a specimen, a specimen stage for supporting the specimen, a fluorescence receiver for receiving fluorescence generated by focus of the phase-modulated light to the specimen, a control unit for displaying a first CGH on the spatial light modulator, and a correction unit for correcting the first CGH. The correction unit comprises a receiver-specific sensitivity information storage preliminarily acquiring and storing sensitivity information per reception position specific to the fluorescence receiver, and a second hologram generator for correcting the first CGH, based on intensities of the fluorescence and the sensitivity information, to generate a second CGH. The control unit displays the second CGH on the spatial light modulator.
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Inventors:
Naoya Matsumoto
Application Number:
JP2015504332A
Publication Date:
February 07, 2018
Filing Date:
March 04, 2014
Export Citation:
Assignee:
Hamamatsu Photonics Co., Ltd.
International Classes:
G02B21/06; G01N21/64; G03H1/08
Domestic Patent References:
| JP2009300589A | ||||
| JP2011128572A | ||||
| JP10186283A | ||||
| JP2005292662A |
Foreign References:
| WO2004017069A1 | ||||
| US20100142014 |
Other References:
Nikolenko V et al.,SLM microscopy: scanless two-photon imaging and photostimulation with spatial light modulators,Frontiers in Neural Circuits,2008年12月19日,Vol.2, No.5,pp.1-14
Shao Y et al.,Ultrafast, large-field multiphoton microscopy based on anacousto-optic deflector and a spatial light,Optics Letters,2012年 7月 1日,Vol.37, No.13,pp.2532-2534
Shao Y et al.,Ultrafast, large-field multiphoton microscopy based on anacousto-optic deflector and a spatial light,Optics Letters,2012年 7月 1日,Vol.37, No.13,pp.2532-2534
Attorney, Agent or Firm:
Yoshiki Hasegawa
Yoshiki Kuroki
Kenichi Shibayama
Yoshiki Kuroki
Kenichi Shibayama