Title:
An improvement of the quantitive nuclease protection assaying (qNPA) method and a quantitive nuclease protection sequence determination (qNPS) method
Document Type and Number:
Japanese Patent JP5951755
Kind Code:
B2
Abstract:
The present disclosure provides an improvement to quantitative Nuclease Protection Assay (qNPA) and quantitative Nuclease Protection Sequencing (qNPS) methods. The disclosed methods use nuclease protection probes (NPPs) that include 5′-end and/or 3-end flanking sequences, which provide a universal hybridization and/or amplification sequence. The disclosed methods can be used to sequence or detect target nucleic acid molecules, such as those present in fixed or insoluble samples.
Inventors:
Selegman, blues
Thompson, Debra
Bashchek, tom
Gordon, Debra A.
Thompson, Debra
Bashchek, tom
Gordon, Debra A.
Application Number:
JP2014509323A
Publication Date:
July 13, 2016
Filing Date:
April 26, 2012
Export Citation:
Assignee:
H.G. Molecular Diagnostics, Incorporated
International Classes:
C12Q1/68; C12N15/09
Foreign References:
| US20080076121 | ||||
| WO2008121927A1 |
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto