Title:
AGENT FOR PROMOTING PROLIFERATION OF MESENCHYMAL STEM CELLS, AGENT FOR PROMOTING CARTILAGE DIFFERENTIATION OF MESENCHYMAL STEM CELLS, MESENCHYMAL-STEM-CELL PREPARATION METHOD, CHONDROCYTIC-CELL PREPARATION METHOD AND MESENCHYMAL-STEM-CELL CULTURE MEDIUM
Document Type and Number:
WIPO Patent Application WO/2016/125582
Kind Code:
A1
Abstract:
As alternatives to conventional mesenchymal-stem-cell preparation methods and methods for preparing chondrocytic cells from mesenchymal stem cell, provided are a novel mesenchymal-stem-cell preparation method that achieves excellent efficiency of promoting proliferation of mesenchymal stem cells, a chondrocytic-cell preparation method that achieves excellent efficiency of promoting differentiation of mesenchymal stem cells into chondrocytic cells, an agent for promoting proliferation of mesenchymal stem cells, an agent for promoting cartilage differentiation of mesenchymal stem cells, and a mesenchymal-stem-cell culture medium that can be used for preparation of such mesenchymal stem cells. The mesenchymal-stem-cell preparation method includes a step in which a mesenchymal-stem-cell culture medium is supplemented with interleukin, and mesenchymal stem cells are cultured in the interleukin-supplemented mesenchymal-stem-cell culture medium. Furthermore, the chondrocytic-cell preparation method includes a step in which mesenchymal stem cells and interleukin are brought into contact with each other, and a step in which cartilage differentiation of the mesenchymal stem cells is induced.
Inventors:
SEKIYA ICHIRO (JP)
MUNETA TAKESHI (JP)
MUNETA TAKESHI (JP)
Application Number:
PCT/JP2016/051447
Publication Date:
August 11, 2016
Filing Date:
January 19, 2016
Export Citation:
Assignee:
NAT UNIV CORP TOKYO MED & DENT (JP)
International Classes:
C12N5/0775; A61K35/12; A61K35/32; A61L27/00; A61P19/02; A61P43/00; C12N5/077
Other References:
LIU, X. ET AL.: "Rescue of proinflammatory cytokine-inhibited chondrogenesis by the antiarthritic effect of melatonin in synovium mesenchymal stem cells via suppression of reactive oxygen species and matrix metalloproteinases.", FREE RADIC. BIOL. MED., vol. 68, March 2014 (2014-03-01), pages 234 - 246, ISSN: 0891-5849
ANDO, W. ET AL.: "Ovine synovial membrane- derived mesenchymal progenitor cells retain the phenotype of the original tissue that was exposed to in-vivo inflammation: evidence for a suppressed chondrogenic differentiation potential of the cells.", INFLAMM. RES., vol. 61, no. 6, June 2012 (2012-06-01), pages 599 - 608, ISSN: 1023-3830
KAZUNORI SHIMOMURA ET AL.: "Hito Katsumaku Yurai Kansaibo o Mochiita Sanjigen Baiyo Saibo eno Rikigaku Shigeki to IL -1beta Shigeki: Saibo Keitai, Matrix Bunkai Koso Idenshi Hatsugen no Kento", THE JOURNAL OF THE JAPANESE ORTHOPAEDIC ASSOCIATION, vol. 83, no. 8, August 2009 (2009-08-01), pages S1130, ISSN: 0021-5325
WARNAWIN, E. ET AL.: "Preservation of chondrogenic potential of mesenchymal stem cells isolated from osteoarthritic patients during proliferation in response to platelet- derived growth factor (PDGF).", CENT. EUR. J. IMMUNOL., vol. 30, no. 1-2, 2005, pages 26 - 31, ISSN: 1426-3912
LI, D. ET AL.: "Biological characteristics of human placental mesenchymal stem cells and their proliferative response to various cytokines.", CELLS TISSUES ORGANS., vol. 186, no. 3, July 2007 (2007-07-01), pages 169 - 179, ISSN: 1422-6405
ANDO, W. ET AL.: "Ovine synovial membrane- derived mesenchymal progenitor cells retain the phenotype of the original tissue that was exposed to in-vivo inflammation: evidence for a suppressed chondrogenic differentiation potential of the cells.", INFLAMM. RES., vol. 61, no. 6, June 2012 (2012-06-01), pages 599 - 608, ISSN: 1023-3830
KAZUNORI SHIMOMURA ET AL.: "Hito Katsumaku Yurai Kansaibo o Mochiita Sanjigen Baiyo Saibo eno Rikigaku Shigeki to IL -1beta Shigeki: Saibo Keitai, Matrix Bunkai Koso Idenshi Hatsugen no Kento", THE JOURNAL OF THE JAPANESE ORTHOPAEDIC ASSOCIATION, vol. 83, no. 8, August 2009 (2009-08-01), pages S1130, ISSN: 0021-5325
WARNAWIN, E. ET AL.: "Preservation of chondrogenic potential of mesenchymal stem cells isolated from osteoarthritic patients during proliferation in response to platelet- derived growth factor (PDGF).", CENT. EUR. J. IMMUNOL., vol. 30, no. 1-2, 2005, pages 26 - 31, ISSN: 1426-3912
LI, D. ET AL.: "Biological characteristics of human placental mesenchymal stem cells and their proliferative response to various cytokines.", CELLS TISSUES ORGANS., vol. 186, no. 3, July 2007 (2007-07-01), pages 169 - 179, ISSN: 1422-6405
Attorney, Agent or Firm:
SHOBAYASHI, Masayuki (JP)
Right wood Masayuki (JP)
Right wood Masayuki (JP)
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