Title:
ARTIFICIAL CHROMOSOME
Document Type and Number:
WIPO Patent Application WO/1998/012336
Kind Code:
A1
Abstract:
An artificial mammalian chromosome, more specifically, a clone containing a mammalian centromere sequence and a DNA replication origin and having mammalian telomere sequences added to both ends is provided by preparing a library of CEPH artificial yeast chromosomes containing a human genome inserted thereinto, identifying clones holding a repetitive human alphoid sequence from this library, and further preparing a yeast strain which adds mammalian telomere sequences to the ends of the chromosome.
Inventors:
ISHIKAWA FUYUKI (JP)
HASEGAWA MAMORU (JP)
HASEGAWA MAMORU (JP)
Application Number:
PCT/JP1997/003305
Publication Date:
March 26, 1998
Filing Date:
September 18, 1997
Export Citation:
Assignee:
DNAVEC RESEARCH INC (JP)
ISHIKAWA FUYUKI (JP)
HASEGAWA MAMORU (JP)
ISHIKAWA FUYUKI (JP)
HASEGAWA MAMORU (JP)
International Classes:
C12N1/19; C12N9/12; C12N15/81; C12N15/85; (IPC1-7): C12N15/85; C12N1/19; C12N9/12
Other References:
TAYLOR S. S., LARIN Z., SMITH C. T.: "ADDITION OF FUNCTIONAL HUMAN TELOMERES TO YACS.", HUMAN MOLECULAR GENETICS, OXFORD UNIVERSITY PRESS, GB, vol. 03., no. 08., 1 August 1994 (1994-08-01), gb, pages 1383 - 1386., XP000601479, ISSN: 0964-6906, DOI: 10.1093/hmg/3.8.1383
AUTEXIER C., GREIDER C. W.: "FUNCTIONAL RECONSTITUTION OF WILD-TYPE AND MUTANT TETRAHYMENA TELOMERASE.", GENES AND DEVELOPMENT., COLD SPRING HARBOR LABORATORY PRESS, PLAINVIEW, NY., US, vol. 08., no. 05., 1 March 1994 (1994-03-01), US, pages 563 - 575., XP002004672, ISSN: 0890-9369
MOYZIS R. K., ET AL.: "A HIGHLY CONSERVED REPETITIVE DNA SEQUENCE, (TTAGGG)N, PRESENT AT THE TELOMERES OF HUMAN CHROMOSOMES.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 85., 1 September 1988 (1988-09-01) - 1 September 1988 (1988-09-01), US, pages 6622 - 6626., XP002030776, ISSN: 0027-8424, DOI: 10.1073/pnas.85.18.6622
See also references of EP 0959134A4
AUTEXIER C., GREIDER C. W.: "FUNCTIONAL RECONSTITUTION OF WILD-TYPE AND MUTANT TETRAHYMENA TELOMERASE.", GENES AND DEVELOPMENT., COLD SPRING HARBOR LABORATORY PRESS, PLAINVIEW, NY., US, vol. 08., no. 05., 1 March 1994 (1994-03-01), US, pages 563 - 575., XP002004672, ISSN: 0890-9369
MOYZIS R. K., ET AL.: "A HIGHLY CONSERVED REPETITIVE DNA SEQUENCE, (TTAGGG)N, PRESENT AT THE TELOMERES OF HUMAN CHROMOSOMES.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 85., 1 September 1988 (1988-09-01) - 1 September 1988 (1988-09-01), US, pages 6622 - 6626., XP002030776, ISSN: 0027-8424, DOI: 10.1073/pnas.85.18.6622
See also references of EP 0959134A4
Attorney, Agent or Firm:
Shimizu, Hatsushi (6th floor 1-1-1, Oroshi-mach, Tsuchiura-shi Ibaraki 300, JP)
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