Title:
ASSAY SYSTEM FOR SIMULTANEOUS DETECTION AND MEASUREMENT OF MULTIPLE MODIFIED CELLULAR PROTEINS
Document Type and Number:
WIPO Patent Application WO2002071067
Kind Code:
A9
Abstract:
A method and kit for simultaneous detection and/or determination of a plurality of modified proteins in a sample. The method comprises: a) contacting the sample under mild protein denaturation conditions with a plurality of first antibodies capable of binding to a specific target protein, the first antibodies being immobilized on solid support material, each first antibody being differentiable from others by a differentiation parameter, whereby the first antibodies bind to respective target proteins present in the sample; b) removing unbound materials from the locus of the first antibodies; c) contacting the materials from step (b) with one or more second antibodies, each of which is specific to a class or subclass of modified proteins or with a plurality of second antibodies, each of which is specific to a modified protein, so as to bind the second antibody or antibodies to modified proteins in the sample; and d) detecting and/or determining a plurality of modified proteins in the sample.
Inventors:
NGUYEN QUAN (US)
SONG YONG (US)
SONG YONG (US)
Application Number:
PCT/US2002/007130
Publication Date:
January 29, 2004
Filing Date:
March 06, 2002
Export Citation:
Assignee:
BIO RAD LABORATORIES (US)
NGUYEN QUAN (US)
SONG YONG (US)
NGUYEN QUAN (US)
SONG YONG (US)
International Classes:
G01N21/64; G01N21/78; G01N33/53; G01N33/543; G01N33/545; G01N33/552; G01N33/68; (IPC1-7): G01N33/53
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