Title:
BISPECIFIC ANTIBODY TARGETING HER2 AND PD-L1, PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/236844
Kind Code:
A1
Abstract:
Disclosed in the present invention are a bispecific antibody targeting HER2 and PD-L1, a preparation method therefor and a use thereof. The bispecific antibody comprises a first protein functional region targeting HER2 and a second protein functional region targeting PD-L1. The first protein functional region comprises VL and VH, and the VL comprises three complementary determining regions, namely LCDR1, LCDR2 and LCDR3 contained in the sequence as shown in SEQ ID NO: 3, and the VH comprises three complementary determining regions, namely HCDR1, HCDR2 and HCDR3 contained in the sequence as shown in SEQ ID NO: 4. The second protein functional region comprises VHH, and the VHH comprises three complementary determining regions, namely CDR1, CDR2 and CDR3 contained in the sequence as shown in SEQ ID NO: 2. The bispecific antibody of the present invention has good purity and thermal stability, the binding activity of two targets is close to that of corresponding monoclonal antibodies, the activity of blocking the PD-1/PD-L1 is equivalent to that of the corresponding monoclonal antibody, and the activity of inhibiting SK-BR-3 cell proliferation is equivalent to that of the corresponding monoclonal antibody, and the bispecific antibody shows good ADCC activity and in-vivo tumor inhibition activity at the same time.
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Inventors:
LIU CHANJUAN (CN)
LANG GUOJUN (CN)
FU ZHIXIANG (CN)
XU CAIYUN (CN)
SI YUANQING (CN)
CAO JINGLI (CN)
ZHANG ZHEN (CN)
YAN RUN (CN)
LANG GUOJUN (CN)
FU ZHIXIANG (CN)
XU CAIYUN (CN)
SI YUANQING (CN)
CAO JINGLI (CN)
ZHANG ZHEN (CN)
YAN RUN (CN)
Application Number:
PCT/CN2023/097835
Publication Date:
December 14, 2023
Filing Date:
June 01, 2023
Export Citation:
Assignee:
SANYOU BIOPHARMACEUTICALS CO LTD (CN)
International Classes:
C07K16/46; A61K39/395; A61P35/00; C12N15/11; C12N15/63; G01N33/574; G01N33/577
Domestic Patent References:
| WO2021244328A1 | 2021-12-09 | |||
| WO2019155408A1 | 2019-08-15 | |||
| WO2019062755A1 | 2019-04-04 |
Foreign References:
| CN113527499A | 2021-10-22 | |||
| CN111848800A | 2020-10-30 | |||
| CN113227151A | 2021-08-06 |
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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