Title:
CELL LYSIS BUFFER SEPARATION APPARATUS FOR PREPARING POLYPEPTIDE, AND SYSTEM AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/179551
Kind Code:
A1
Abstract:
A cell lysis buffer separation apparatus for preparing a polypeptide, and a system and the application thereof. The system comprises a polypeptide enrichment module, wherein the polypeptide enrichment module comprises a cell lysis buffer separation apparatus, and the cell lysis buffer separation apparatus comprises a multi-filtration device and a waste discharge pipeline, the waste discharge pipeline being connected to the multi-filtration device. The system can perform multi-filtration treatment on a lysis buffer. Therefore, the yield of target protein can be increased; and a waste liquid obtained by means of the system contains almost no toxin polypeptide precursor with low toxicity, and has very little toxicity to an operating environment, and same can be directly discharged after simple disinfection treatment, thereby greatly reducing production cost and promoting large-scale industrial production of polypeptide under the conditions of ensuring the safety of the environment and the safety of operators.
Inventors:
ZHANG YAN (CN)
Application Number:
PCT/CN2022/077629
Publication Date:
September 01, 2022
Filing Date:
February 24, 2022
Export Citation:
Assignee:
CHONGQING CLARUVIS PHARMACEUTICAL CO LTD (CN)
International Classes:
B01D36/00; C12M1/00
Foreign References:
| US20030170810A1 | 2003-09-11 | |||
| CN215742274U | 2022-02-08 | |||
| CN214654579U | 2021-11-09 | |||
| CN102167726A | 2011-08-31 | |||
| CN1397344A | 2003-02-19 | |||
| CN101415482A | 2009-04-22 | |||
| CN109971776A | 2019-07-05 |
Other References:
"Molecular Cloning A Laboratory Manua", 1989, COLD SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
BEIJING LINKTEC IP LAW FIRM (SPECIAL GENERAL PARTNERSHIP) (CN)
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