Title:
COMPOSITE MAGNETIC NANOMATERIAL BASED ON DNA TETRAHEDRON, PREPARATION THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/156677
Kind Code:
A1
Abstract:
The present invention relates to the technical field of functionalized magnetic nanomaterials. Provided are a composite magnetic nanomaterial based on a DNA tetrahedron, a preparation therefor and use thereof. The preparation method comprises: synthesizing a DNA tetrahedron by means of a self-assembly reaction of single-stranded DNA, loading magnetic nanoparticles on the surface of molybdenum disulfide particles, modifying gold nanoparticles on exposed active sulfur atoms of the molybdenum disulfide particles, modifying the DNA tetrahedron on the gold nanoparticles, and connecting a protein antibody to the DNA tetrahedron in an incubating manner. The material prepared by the method of the present invention is used for enriching and detecting low-abundance proteins in a serum, and specific low-abundance proteins in the serum can be efficiently and selectively enriched by means of the specific reaction between an antigen and an antibody. In addition, the material takes a magnetic nanomaterial as a matrix, thus has the characteristic of being simple, convenient and fast to use, the treatment time of the complex matrix in the serum is greatly shortened, and highly efficient and highly selective enrichment of the low-abundance proteins in the complex matrix can be realized.
Inventors:
ZHAI RUI (CN)
CHU ZHANYING (CN)
ZHU MANMAN (CN)
ZHAO YANG (CN)
GONG XIAOYUN (CN)
XIE JIE (CN)
WU LIQING (CN)
JIANG YOU (CN)
DAI XINHUA (CN)
FANG XIANG (CN)
CHU ZHANYING (CN)
ZHU MANMAN (CN)
ZHAO YANG (CN)
GONG XIAOYUN (CN)
XIE JIE (CN)
WU LIQING (CN)
JIANG YOU (CN)
DAI XINHUA (CN)
FANG XIANG (CN)
Application Number:
PCT/CN2022/072553
Publication Date:
July 28, 2022
Filing Date:
January 18, 2022
Export Citation:
Assignee:
NAT INST METROLOGY CHINA (CN)
International Classes:
G01N33/68; H01F1/00; H01F41/02
Foreign References:
| CN112924695A | 2021-06-08 | |||
| CN110779789A | 2020-02-11 | |||
| CN108562738A | 2018-09-21 | |||
| CN106568936A | 2017-04-19 |
Attorney, Agent or Firm:
BEIJING JINZHIPUHUA INTELLECTUAL PROPERTY AGENCY CO.LTD (CN)
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