Title:
ENHANCER ELEMENTS FOR INCREASED TRANSLATION IN PLANT PLASTIDS
Document Type and Number:
WIPO Patent Application WO2000003017
Kind Code:
A3
Abstract:
Provided are methods for increasing the production of protein in a plant cell by transforming plastids of plant cells with a construct comprising a promoter functional in a plant plastid, a ribosome binding site, DNA sequence of interest and a transcription termination region, and growing plant cells comprising the transformed plastids under conditions wherein the DNA encoding sequence is transcribed in the plastid. Also provided are methods for increasing protein production by fusing a coding sequence to a gene of interest to a secondary protein for cleavage or targetting of the protein of interest within the plastid, whereby high levels of expression of protein is achieved.
Inventors:
MCBRIDE KEVIN E (US)
STAUB JEFFREY M (US)
STAUB JEFFREY M (US)
Application Number:
PCT/US1999/015713
Publication Date:
July 06, 2000
Filing Date:
July 08, 1999
Export Citation:
Assignee:
CALGENE LLC (US)
MCBRIDE KEVIN E (US)
STAUB JEFFREY M (US)
MCBRIDE KEVIN E (US)
STAUB JEFFREY M (US)
International Classes:
A01H5/00; A01H5/10; C07K14/61; C07K14/81; C12N5/10; C12N9/10; C12N15/09; C12N15/15; C12N15/29; C12N15/31; C12N15/33; C12N15/82; (IPC1-7): C12N15/15; C12N15/18; C12N15/54; C12N15/62; C12N15/82; C12N5/10; A01H5/00; A01H5/10
Domestic Patent References:
| WO1995016783A1 | 1995-06-22 | |||
| WO1998011235A2 | 1998-03-19 | |||
| WO1995024493A1 | 1995-09-14 | |||
| WO1992004449A1 | 1992-03-19 |
Foreign References:
| EP0293358A2 | 1988-11-30 | |||
| US5693507A | 1997-12-02 |
Other References:
MCBRIDE ET AL: "Amplification of a chimeric Bacillus gene in chloroplasts leads to an extraordinary level of an insecticial protein in tobacco", BIO/TECHNOLOGY,US,NATURE PUBLISHING CO. NEW YORK, vol. 13, 13 April 1995 (1995-04-13), pages 362-365, XP002090404, ISSN: 0733-222X
DANIELL ET AL: "Containment of herbicide resistance through genetic engineering of the chloroplast genome", NATURE BIOTECHNOLOGY,US,NATURE PUBLISHING, vol. 16, April 1998 (1998-04-01), pages 345-348, XP002090409, ISSN: 1087-0156
DANIELL ET AL: "Containment of herbicide resistance through genetic engineering of the chloroplast genome", NATURE BIOTECHNOLOGY,US,NATURE PUBLISHING, vol. 16, April 1998 (1998-04-01), pages 345-348, XP002090409, ISSN: 1087-0156
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