Title:
ENZYME CHIP AND UTILIZATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2005/071099
Kind Code:
A1
Abstract:
It is intended to provide an enzyme chip which makes it possible to simultaneously and efficiency assay a plural number of enzyme activities and a method of enzyme-specific and activity-dependent enzyme activity assay with the use of this enzyme chip. It is also intended to provide a high throughput method of screening an enzyme activity-controlling substance by which a substance capable of controlling enzyme activity can be efficiently searched for. It is further intended to provide an automated system for carrying out the above methods. A cysteine protease-immobilized chip for assaying enzyme activity wherein a plural number of cysteine proteases differing from each other are provided respectively at a plural number of positions differing from each other on a substrate; and a method of specifically and activity-dependently assaying the biological activity of a cysteine protease with the use of an affinity label.
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Inventors:
FUNERIU DANIEL PETRU (JP)
EPPINGER JOERG (JP)
MIYAKE MASATO (JP)
MIYAKE JUN (JP)
EPPINGER JOERG (JP)
MIYAKE MASATO (JP)
MIYAKE JUN (JP)
Application Number:
PCT/JP2004/019247
Publication Date:
August 04, 2005
Filing Date:
December 22, 2004
Export Citation:
Assignee:
NAT INST OF ADVANCED IND SCIEN (JP)
FUNERIU DANIEL PETRU (JP)
EPPINGER JOERG (JP)
MIYAKE MASATO (JP)
MIYAKE JUN (JP)
FUNERIU DANIEL PETRU (JP)
EPPINGER JOERG (JP)
MIYAKE MASATO (JP)
MIYAKE JUN (JP)
International Classes:
C12M1/34; C12Q1/00; C12Q1/37; G01N33/15; G01N33/50; (IPC1-7): C12Q1/37; C12M1/34; G01N33/15; G01N33/50
Domestic Patent References:
| WO2002088296A1 | 2002-11-07 |
Foreign References:
| JP2004093331A | 2004-03-25 | |||
| JP2003207505A | 2003-07-25 | |||
| JP2002139493A | 2002-05-17 |
Other References:
EPPINGER J. ET AL: "Enzyme microarrays: On-chip determination of inhibition constants based on affinity-label detection of enzymatic activity", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 43, no. 29, 19 July 2004 (2004-07-19), pages 3806 - 3810, XP009076269
EPPINGER J. ET AL: "Enzyme Microarrays: On-Chip Determination of Inhibition Constants Based on Affinity-Label Detection of Enzymatic Activity", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 43, no. 34, 27 August 2004 (2004-08-27), pages 4389
MARTIN K. ET AL: "Quantitative analysis of protein phosphorylation status and protein kinase activity on microarrays using novel fluorescent phosphorylation sensor dye", PROTEOMICS., vol. 3, no. 7, July 2003 (2003-07-01), pages 1244 - 1255, XP009048093
EPPINGER J. ET AL: "Enzyme Microarrays: On-Chip Determination of Inhibition Constants Based on Affinity-Label Detection of Enzymatic Activity", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 43, no. 34, 27 August 2004 (2004-08-27), pages 4389
MARTIN K. ET AL: "Quantitative analysis of protein phosphorylation status and protein kinase activity on microarrays using novel fluorescent phosphorylation sensor dye", PROTEOMICS., vol. 3, no. 7, July 2003 (2003-07-01), pages 1244 - 1255, XP009048093
Attorney, Agent or Firm:
Kitamura, Shuichiro (Nakanoshima 2-chome Kita-k, Osaka-shi Osaka, JP)
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