Title:
GENETIC ENGINEERING MODIFIED DUAL-TARGET CHIMERIC ANTIGEN RECEPTOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/125277
Kind Code:
A1
Abstract:
A genetic engineering modified dual-target chimeric antigen receptor, a host cell for expressing the dual-target antigen receptor, and the use thereof in preventing or treating malignant tumors/solid tumors. With regard to the up-regulated expression of PD-L1 of a tumor cell and an immune cell occurring after the tumor cell is exposed in serous cavity hydrops, provided is a genetic engineering modified dual-target chimeric antigen receptor, and a host cell thereof. The dual-target chimeric antigen receptor is competitively bond to PD-L1 to convert an inhibition signal of PD-L1 into an activated signal, and improve the killing activity of a T cell. Meanwhile, 4-1BB introduced at the downstream of the receptor can promote T cell proliferation and activation.
Inventors:
WANG YONGSHENG (CN)
LI DAN (CN)
YANG QIYU (CN)
MA QIZHI (CN)
LI DAN (CN)
YANG QIYU (CN)
MA QIZHI (CN)
Application Number:
PCT/CN2019/117601
Publication Date:
June 25, 2020
Filing Date:
November 12, 2019
Export Citation:
Assignee:
WEST CHINA HOSPITAL SICHUAN UNIV (CN)
International Classes:
C07K19/00; A61K35/17; A61P35/04; C12N5/10; C12N15/63
Domestic Patent References:
| WO2017075440A1 | 2017-05-04 | |||
| WO2017080377A1 | 2017-05-18 |
Foreign References:
| CN107325185A | 2017-11-07 | |||
| CN105384825A | 2016-03-09 | |||
| CN109485732A | 2019-03-19 |
Other References:
ZHANG YUN, JI XILUAN, LUO ZHAOXIA, YANG SHUN, SHANG YANHONG, XIE LIANG, JIA YOUCHAO, LI JIEMING : "Experimental Study of Dual Chimeric Antigen Receptor T Cells Targeted Therapy for Acute Myeloid Leukemia", JOURNAL OF INTERNATIONAL ONCOLOGY, vol. 45, no. 7, 31 July 2018 (2018-07-31), CN, pages 385 - 390, XP009522197, ISSN: 1673-422X, DOI: 10.3760/cma.j.issn.1673-422X.2018.07.001
LYDIA MEDER; PHILIPP SCHULDT; MARTIN THELEN; ANNA SCHMITT; FELIX DIETLEIN; SEBASTIAN KLEIN; SVEN BORCHMANN; KERSTIN WENNHOLD; IGNA: "Combined VEGF and PD-L1 Blockade Displays Synergistic Treatment Effects in an Autochthonous Mouse Model of Small Cell Lung Cancer", CANCER RESEARCH, vol. 78, no. 15, 31 August 2018 (2018-08-31), pages 4270 - 4281, XP055702639, ISSN: 0008-5472, DOI: 10.1158/0008-5472.CAN-17-2176
LIZA B JOHN; MICHAEL H KERSHAW; PHILLIP K DARCY: "Blockade of PD-1 immunosuppression boosts CAR T- cell therapy", ONCOLMMUNOLOGY, vol. 2, no. 10, e26266, 10 October 2013 (2013-10-10), pages 1 - 4, XP055370472, DOI: 10.4161/onci.26286
SAMBROOK JRUSSELL D.W.: "Molecular Cloning:A laboratory manual", 2001, SPRING HARBOR LABORATORY PRESS
LYDIA MEDER; PHILIPP SCHULDT; MARTIN THELEN; ANNA SCHMITT; FELIX DIETLEIN; SEBASTIAN KLEIN; SVEN BORCHMANN; KERSTIN WENNHOLD; IGNA: "Combined VEGF and PD-L1 Blockade Displays Synergistic Treatment Effects in an Autochthonous Mouse Model of Small Cell Lung Cancer", CANCER RESEARCH, vol. 78, no. 15, 31 August 2018 (2018-08-31), pages 4270 - 4281, XP055702639, ISSN: 0008-5472, DOI: 10.1158/0008-5472.CAN-17-2176
LIZA B JOHN; MICHAEL H KERSHAW; PHILLIP K DARCY: "Blockade of PD-1 immunosuppression boosts CAR T- cell therapy", ONCOLMMUNOLOGY, vol. 2, no. 10, e26266, 10 October 2013 (2013-10-10), pages 1 - 4, XP055370472, DOI: 10.4161/onci.26286
SAMBROOK JRUSSELL D.W.: "Molecular Cloning:A laboratory manual", 2001, SPRING HARBOR LABORATORY PRESS
Attorney, Agent or Firm:
CHENGDU HONGQIAO PATENT LAW OFFICE (CN)
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