Title:
HIGH THROUGHPUT ASSAY BASED ON THE USE OF A POLYPEPTIDE BINDING PAIR
Document Type and Number:
WIPO Patent Application WO2000050902
Kind Code:
A3
Abstract:
This invention relates to a method for analysing a sample comprising: immobilising a polypeptide to a physical support; contacting the immobilised polypeptide with a test sample which may contain an agent capable of modifying the immobilised polypeptide; contacting the immobilised polypeptide with a binding partner polypeptide, wherein the binding of this partner polypeptide to the immobilised polypeptide is at least partly dependent on the modification state of the immobilised polypeptide; and measuring the association of the binding partner polypeptide to the immobilised polypeptide.
Inventors:
COLYER JOHN
CRAIG ROGER KINGDON
MASCHIO ANTONIO
MEZNA MOKDAD
CRAIG ROGER KINGDON
MASCHIO ANTONIO
MEZNA MOKDAD
Application Number:
PCT/GB2000/000669
Publication Date:
December 14, 2000
Filing Date:
February 25, 2000
Export Citation:
Assignee:
FLUORESCIENCE LTD (GB)
International Classes:
C12Q1/25; C12Q1/37; G01N33/573; G01N33/68; (IPC1-7): G01N33/543; C07K14/00; C12Q1/25; C12Q1/34; C12Q1/42
Domestic Patent References:
| WO1996035806A1 | 1996-11-14 | |||
| WO1996020211A1 | 1996-07-04 | |||
| WO1998039471A1 | 1998-09-11 | |||
| WO1996021040A1 | 1996-07-11 | |||
| WO1998020024A1 | 1998-05-14 | |||
| WO1999011774A1 | 1999-03-11 |
Foreign References:
| EP0392808A2 | 1990-10-17 |
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