Title:
HUMAN MEKK PROTEINS, CORRESPONDING NUCLEIC ACID MOLECULES, AND USES THEREFOR
Document Type and Number:
WIPO Patent Application WO1999047686
Kind Code:
A3
Abstract:
Isolated nucleic acid molecules encoding human MEKK proteins, and isolated MEKK proteins, are provided. The invention further provides antisense nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals carrying a human MEKK transgene. The invention further provides human MEKK fusion proteins and anti-human MEKK antibodies. Methods of using the human MEKK proteins and nucleic acid molecules of the invention are also disclosed, including methods for detecting human MEKK activity in a biological sample, methods of modulating human MEKK activity in a cell, and methods for identifying agents that modulate the activity of human MEKK.
Inventors:
JOHNSON GARY L (US)
Application Number:
PCT/US1999/005556
Publication Date:
January 27, 2000
Filing Date:
March 15, 1999
Export Citation:
Assignee:
CADUS PHARMACEUTICAL CORP (US)
JOHNSON GARY L (US)
JOHNSON GARY L (US)
International Classes:
C12N9/12; C12N15/54; (IPC1-7): C12N15/54; A01K67/027; A61K31/00; C07K16/40; C07K19/00; C12N5/10; C12N9/12; C12N15/62; G01N33/68
Domestic Patent References:
| WO1997035014A1 | 1997-09-25 | |||
| WO1995028421A1 | 1995-10-26 |
Other References:
DATABASE EMBL NUCLEOTIDE SEQU 1 January 1900 (1900-01-01), XP002108368, Database accession no. AF042838
XU S. ET AL.: "Cloning of rat MEK kinase 1 cDNA reveals an endogenous membrane-associated 195 kDa protein with a large regulatory domain.", PROC. NATL. ACAD. SCI. USA, vol. 93, May 1996 (1996-05-01), pages 5291 - 5295, XP002108363
WIDMANN C. ET AL.: "Potentiation of apoptosis by low dose stress stimuli in cells expressing activated MEK kinase 1.", ONCOGENE, vol. 15, no. 20, November 1997 (1997-11-01), pages 2439 - 2447, XP002108366
FANGER G. R. ET AL.: "MEK kinases are regulated by EGF and selectively interact with Rac/Cdc42.", EMBO JOURNAL, vol. 16, no. 16, 1997, pages 4961 - 4972, XP002108750
CARDONE M. H. ET AL.: "The regulation of Anoikis: MEKK-1 activation requires cleavage by caspases.", CELL, vol. 90, 25 July 1997 (1997-07-25), pages 315 - 323, XP002108365
LANGE-CARTER C. A. ET AL.: "A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf.", SCIENCE, vol. 260, 16 April 1993 (1993-04-16), pages 315 - 319, XP002108364
XIA Y. ET AL.: "JNKK1 organizes a MAP kinase module through specific and sequential interactions with the upstream and downstream components mediated by its amino-terminal extension.", GENES AND DEVELOPMENT, vol. 12, no. 21, 1 November 1998 (1998-11-01), pages 3369 - 3381, XP002108751
XU S. ET AL.: "Cloning of rat MEK kinase 1 cDNA reveals an endogenous membrane-associated 195 kDa protein with a large regulatory domain.", PROC. NATL. ACAD. SCI. USA, vol. 93, May 1996 (1996-05-01), pages 5291 - 5295, XP002108363
WIDMANN C. ET AL.: "Potentiation of apoptosis by low dose stress stimuli in cells expressing activated MEK kinase 1.", ONCOGENE, vol. 15, no. 20, November 1997 (1997-11-01), pages 2439 - 2447, XP002108366
FANGER G. R. ET AL.: "MEK kinases are regulated by EGF and selectively interact with Rac/Cdc42.", EMBO JOURNAL, vol. 16, no. 16, 1997, pages 4961 - 4972, XP002108750
CARDONE M. H. ET AL.: "The regulation of Anoikis: MEKK-1 activation requires cleavage by caspases.", CELL, vol. 90, 25 July 1997 (1997-07-25), pages 315 - 323, XP002108365
LANGE-CARTER C. A. ET AL.: "A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf.", SCIENCE, vol. 260, 16 April 1993 (1993-04-16), pages 315 - 319, XP002108364
XIA Y. ET AL.: "JNKK1 organizes a MAP kinase module through specific and sequential interactions with the upstream and downstream components mediated by its amino-terminal extension.", GENES AND DEVELOPMENT, vol. 12, no. 21, 1 November 1998 (1998-11-01), pages 3369 - 3381, XP002108751
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