Title:
INSULIN PRECURSOR PURIFYING METHOD USING ANION EXCHANGE CHROMATOGRAPHY
Document Type and Number:
WIPO Patent Application WO/2020/138953
Kind Code:
A1
Abstract:
The present invention relates to an insulin precursor purifying method for improving the production yield of an insulin precursor by adjusting the pHs of a first buffer solution for equilibrating ion exchange resins and a second buffer solution for eluting an insulin precursor bound to the ion exchange resins. The insulin precursor purifying method according to the present invention is a high-purity and high-yield insulin precursor purifying method for reinforcing, by mean of a suitable pH combination of buffer solutions, the binding force of an insulin precursor to ion exchange resins and enabling the insulin precursor to be effectively eluted thereafter, and is very useful in high-yield insulin production through enzymatic conversion after a purification process.
Inventors:
KIM JINHWAN (KR)
BYUN SEUNGMIN (KR)
SONG JEYOUN (KR)
KIM HYUNUK (KR)
YOON JUNGPIL (KR)
BYUN SEUNGMIN (KR)
SONG JEYOUN (KR)
KIM HYUNUK (KR)
YOON JUNGPIL (KR)
Application Number:
PCT/KR2019/018468
Publication Date:
July 02, 2020
Filing Date:
December 26, 2019
Export Citation:
Assignee:
POLUS INC (KR)
International Classes:
C07K14/62; B01D15/36; C07K1/18
Foreign References:
| US5952461A | 1999-09-14 | |||
| US20060234226A1 | 2006-10-19 | |||
| KR20160030251A | 2016-03-16 | |||
| KR20150084046A | 2015-07-21 | |||
| EP0368187A2 | 1990-05-16 | |||
| US5750497A | 1998-05-12 | |||
| US7615532B2 | 2009-11-10 | |||
| KR20017013921A | 2001-10-30 | |||
| EP0211299A2 | 1987-02-25 | |||
| EP0227938A2 | 1987-07-08 | |||
| EP0229998A2 | 1987-07-29 | |||
| EP0286956A2 | 1988-10-19 | |||
| KR0158197B1 | 1998-12-01 | |||
| US5656722A | 1997-08-12 |
Other References:
ELLIS, S. ET AL.: "The chromatography of growth hormone on cellulose derivatives", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 220, 1956, pages 939 - 949, XP055721249
See also references of EP 3904372A4
See also references of EP 3904372A4
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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