Title:
COMPOSITION FOR CONVERTING INSULIN PRECURSOR INTO INSULIN ENZYME AND METHOD FOR CONVERTING INSULIN PRECURSOR INTO INSULIN BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2020/138952
Kind Code:
A1
Abstract:
The present invention relates to: a composition for an enzyme conversion reaction for converting proinsulin or a proinsulin analogue into insulin or an insulin analogue, the composition containing one or more selected from the group consisting of calcium chloride, glycine and proline, and trypsin and/or carboxypeptidase B; and a method for converting proinsulin or a proinsulin analogue into insulin or an insulin analogue by using the composition. The present invention increases the structural stability of an insulin protein and the stability of the enzyme at the same time so as to significantly improve the enzyme conversion yield and the purity of insulin, thereby being useful in insulin production processes.
Inventors:
KIM JINHWAN (KR)
HYUN SEUNGMIN (KR)
JO MUNJEONG (KR)
KIM HYUNUK (KR)
KIM DONGKYU (KR)
HYUN SEUNGMIN (KR)
JO MUNJEONG (KR)
KIM HYUNUK (KR)
KIM DONGKYU (KR)
Application Number:
PCT/KR2019/018465
Publication Date:
July 02, 2020
Filing Date:
December 26, 2019
Export Citation:
Assignee:
POLUS INC (KR)
International Classes:
C07K14/62; C12P21/00
Foreign References:
| US5457066A | 1995-10-10 | |||
| US20110152507A1 | 2011-06-23 | |||
| KR20110059602A | 2011-06-02 | |||
| KR20170036643A | 2017-04-03 | |||
| KR100202958B1 | 1999-06-15 | |||
| EP0368187A2 | 1990-05-16 | |||
| US5750497A | 1998-05-12 | |||
| US7615532B2 | 2009-11-10 | |||
| KR20017013921A | 2001-10-30 | |||
| EP0211299A2 | 1987-02-25 | |||
| EP0227938A2 | 1987-07-08 | |||
| EP0229998A2 | 1987-07-29 | |||
| EP0286956A2 | 1988-10-19 | |||
| KR0158197B1 | 1998-12-01 | |||
| US5656722A | 1997-08-12 |
Other References:
See also references of EP 3904373A4
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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