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Title:
KIT FOR DETECTING BOVINE LEUKEMIA VIRUS
Document Type and Number:
WIPO Patent Application WO/2021/100668
Kind Code:
A1
Abstract:
[Problem] To provide a kit for detecting bovine leukemia virus, the kit comprising a primer set with which a subject suffering from bovine leukemia virus can be detected with high sensitivity. [Solution] This kit for detecting bovine leukemia virus comprises a primer set comprising a forward primer and a reverse primer, the forward primer being an oligonucleotide comprising a base sequence represented by SEQ ID NO:1 or 4, the reverse primer being an oligonucleotide comprising a base sequence represented by SEQ ID NO: 2 or 5. SEQ ID NO:1: ACCATGGACTTTCACTTCTGG, SEQ ID NO:2: GGCCAAGACAAGGGGAAC, SEQ ID NO:4: CTCCCCAACTTCCCCTTTC, SEQ ID NO:5: ACGAGGGTCTCAGGAGAAG

Inventors:
MIZUTANI TETSUYA (JP)
NUNOMURA YUKA (JP)
YAMAGUCHI TOMOHIRO (JP)
Application Number:
PCT/JP2020/042669
Publication Date:
May 27, 2021
Filing Date:
November 16, 2020
Export Citation:
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Assignee:
CANINE LAB INC (JP)
International Classes:
C12N15/00; C12Q1/686; C12Q1/6888; G01N33/50
Foreign References:
CN109182602A2019-01-11
Other References:
SOMURA, Y. ET AL.: "Comparison of the copy numbers of bovine leukemia virus in the lymph nodes of cattle with enzootic bovine leukosis and cattle with latent infection", ARCH VIROL, vol. 159, 12 June 2014 (2014-06-12), pages 2693 - 2697, XP035396555, DOI: 10.1007/s00705-014-2137-9
JIMBA, M. ET AL.: "BLV-CoCoMo-qPCR: a useful tool for evaluating bovine leukemia virus infection status", BMC VETERINARY RESEARCH, vol. 8, no. 1, 167, 21 September 2012 (2012-09-21), pages 1 - 12, XP021122388
JIMBA, M. ET AL.: "BLV-CoCoMo-qPCR: Quantitation of bovine leukemia virus proviral load using the CoCoMo algorithm", RETROVIROLOGY, vol. 7, no. 1, 2010, pages 91, XP021079651
MARSOLAIS, G. ET AL.: "Importance of primer selection in the application of PCR technology to the diagnosis of bovine leukemia virus", J VET DIAGN INVEST, vol. 6, no. 3, July 1994 (1994-07-01), pages 297 - 301, XP055825739
Attorney, Agent or Firm:
HIROSE Takayuki (JP)
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