Title:
LARGE CIRCULAR SENSE MOLECULE ARRAY
Document Type and Number:
WIPO Patent Application WO2004013277
Kind Code:
A3
Abstract:
Large circular (LC)-sense molecules in an array is disclosed. The LC-sense molecules array is combined with cDNA hybridization to detect differences in expression profile between different cells. LC-sense molecules were purified from nonredundant clones with recombinant phagemid and arrayed onto silanized slide glasses. By hybridization of LC-sense array with Cy3 or Cy5-labelled cDNA preparations at 60 °C , 29 up-regulated and 6 down-regulated genes in cancerous liver tissue were detected.
Inventors:
PARK JONG-GU (KR)
LEE YUN-HAN (KR)
LEE YUN-HAN (KR)
Application Number:
PCT/IB2003/004955
Publication Date:
June 03, 2004
Filing Date:
July 25, 2003
Export Citation:
Assignee:
WELGENE INC (KR)
PARK JONG-GU (KR)
LEE YUN-HAN (KR)
PARK JONG-GU (KR)
LEE YUN-HAN (KR)
International Classes:
C12N7/01; C12Q1/68; (IPC1-7): C12Q1/70; C12N7/01; C12N7/02; C12N15/10; C12Q1/68
Domestic Patent References:
| WO1999000520A1 | 1999-01-07 | |||
| WO2001000786A2 | 2001-01-04 |
Foreign References:
| US20020055099A1 | 2002-05-09 |
Other References:
DRMANAC R. ET AL.: "Sequencing by hybridization: towards an automated sequencing of one millions M13 clones arrayed on membranes", ELECTROPHORESIS, vol. 13, no. 8, 1992, pages 566 - 573, XP009011315
DRMANAC S AND DRMANAC R: "Processing of cDNA and genomic kilobase-size clones for massive screening, mapping and sequencing by hydridization", BIOTECHNIQUES, vol. 17, no. 2, 1994, pages 328 - 329, XP009011315
KAINZ P. ET AL.: "A modified primer extension procedure for specific detection of DNA-RNA hybrids on nylon membranes", ANALYTICAL BIOCHEMISTRY, vol. 179, 1989, pages 366 - 370, XP008048074
See also references of EP 1543167A4
DRMANAC S AND DRMANAC R: "Processing of cDNA and genomic kilobase-size clones for massive screening, mapping and sequencing by hydridization", BIOTECHNIQUES, vol. 17, no. 2, 1994, pages 328 - 329, XP009011315
KAINZ P. ET AL.: "A modified primer extension procedure for specific detection of DNA-RNA hybrids on nylon membranes", ANALYTICAL BIOCHEMISTRY, vol. 179, 1989, pages 366 - 370, XP008048074
See also references of EP 1543167A4
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