Title:
METHOD FOR DETECTING AMYLOID-β BOUND TO BRAIN-NEURONAL-CELL-DERIVED EXOSOME IN BLOOD, KIT FOR DETECTION OF AMYLOID-β BOUND TO BRAIN-NEURONAL-CELL-DERIVED EXOSOME IN BLOOD, AND METHOD FOR EVALUATING ACCUMULATION LEVEL OF AMYLOID-β IN BRAIN
Document Type and Number:
WIPO Patent Application WO/2023/080102
Kind Code:
A1
Abstract:
Provided are: a method for detecting amyloid-β bound to a brain-neuronal-cell-derived exosome in blood, the method comprising a step for binding amyloid-β bound to the brain-neuronal-cell-derived exosome in the blood to an amyloid-β specifically binding substance that is labeled with a nucleic acid fragment and is capable of binding specifically to amyloid-β and a step for detecting the amyloid-β specifically binding substance; and a method for evaluating the accumulation level of amyloid-β in the brain, the method comprising a step for detecting amyloid-β that is bound to a brain-neuronal-cell-derived exosome in blood and a step for evaluating the accumulation level of amyloid-β in the brain based on the amount of amyloid-β detected.
Inventors:
MAKINO YOICHI (JP)
HIRASE TAKUMI (JP)
YUYAMA KOHEI (JP)
HIRASE TAKUMI (JP)
YUYAMA KOHEI (JP)
Application Number:
PCT/JP2022/040614
Publication Date:
May 11, 2023
Filing Date:
October 31, 2022
Export Citation:
Assignee:
TOPPAN INC (JP)
UNIV HOKKAIDO NAT UNIV CORP (JP)
UNIV HOKKAIDO NAT UNIV CORP (JP)
International Classes:
C12Q1/682; G01N33/53; G01N33/536
Domestic Patent References:
| WO2020157705A1 | 2020-08-06 | |||
| WO2017200070A1 | 2017-11-23 | |||
| WO2020235607A1 | 2020-11-26 | |||
| WO2018207906A1 | 2018-11-15 | |||
| WO2013054534A1 | 2013-04-18 | |||
| WO2015115635A1 | 2015-08-06 |
Foreign References:
| JP2021180612A | 2021-11-18 |
Other References:
SINGH NETRAPAL, HUANG LIN, WANG DIAN-BING, SHAO NAN, ZHANG XIAN-EN: "Simultaneous Detection of a Cluster of Differentiation Markers on Leukemia-Derived Exosomes by Multiplex Immuno-Polymerase Chain Reaction via Capillary Electrophoresis Analysis", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 92, no. 15, 4 August 2020 (2020-08-04), US , pages 10569 - 10577, XP093063571, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.0c01464
YUYAMA KOHEI, SUN HUI, IGARASHI YASUYUKI, MONDE KENJI, HIRASE TAKUMI, NAKAYAMA MASATO, MAKINO YOICHI: "Immuno-digital invasive cleavage assay for analyzing Alzheimer’s amyloid ß-bound extracellular vesicles", ALZHEIMER'S RESEARCH & THERAPY, vol. 14, no. 1, 3 October 2022 (2022-10-03), pages 140, XP093063574, DOI: 10.1186/s13195-022-01073-w
LAWRENCE RAJENDRAN ET AL.: "Alzheimer's disease (3-amyloid peptides are released in association with exosomes", PROC. NATL. ACAD. SCI. USA, vol. 103, no. 30, 25 July 2006 (2006-07-25), pages 11172 - 11177, XP055447876, DOI: 10.1073/pnas.0603838103
MASSIMO S. FIANDACA: "Identification of preclinical Alzheimer's disease by a profile of pathogenic proteins in neurally derived blood exosomes: A case-control study", ALZHEIMER'S & DEMENTIA, vol. 11, 2015, pages 600 - 607
OLMEDILLAS-LOPEZ S. ET AL.: "Current and Emerging Applications of Droplet Digital PCR in Oncology", MOL DIAGN THER, vol. 21, no. 5, October 2017 (2017-10-01), pages 493 - 510, XP055531296, DOI: 10.1007/s40291-017-0278-8
YUYAMA KOHEI, SUN HUI, IGARASHI YASUYUKI, MONDE KENJI, HIRASE TAKUMI, NAKAYAMA MASATO, MAKINO YOICHI: "Immuno-digital invasive cleavage assay for analyzing Alzheimer’s amyloid ß-bound extracellular vesicles", ALZHEIMER'S RESEARCH & THERAPY, vol. 14, no. 1, 3 October 2022 (2022-10-03), pages 140, XP093063574, DOI: 10.1186/s13195-022-01073-w
LAWRENCE RAJENDRAN ET AL.: "Alzheimer's disease (3-amyloid peptides are released in association with exosomes", PROC. NATL. ACAD. SCI. USA, vol. 103, no. 30, 25 July 2006 (2006-07-25), pages 11172 - 11177, XP055447876, DOI: 10.1073/pnas.0603838103
MASSIMO S. FIANDACA: "Identification of preclinical Alzheimer's disease by a profile of pathogenic proteins in neurally derived blood exosomes: A case-control study", ALZHEIMER'S & DEMENTIA, vol. 11, 2015, pages 600 - 607
OLMEDILLAS-LOPEZ S. ET AL.: "Current and Emerging Applications of Droplet Digital PCR in Oncology", MOL DIAGN THER, vol. 21, no. 5, October 2017 (2017-10-01), pages 493 - 510, XP055531296, DOI: 10.1007/s40291-017-0278-8
Attorney, Agent or Firm:
MATSUNUMA Yasushi et al. (JP)
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