METHOD FOR DETERMINING CELL STATE AND AUTOANALYZER USING SAID METHOD

Title:

METHOD FOR DETERMINING CELL STATE AND AUTOANALYZER USING SAID METHOD

Document Type and Number:

WIPO Patent Application WO/2014/208391

Kind Code:

A1

Abstract:

Provided is a method for noninvasively evaluating the cell state (proliferating, layering, and differentiating) of a cell sheet, which is a biomimetic tissue, when culturing a cell sheet. The method is characterized in that an amino acid analysis is conducted using the culture supernatant of the cell sheet and assessing the cell state by monitoring changes in the concentration of any of five amino acids (Ile, Val, Ser, Leu, and Ala).

Inventors:

SENDA NAOKO (JP)
INOUE YOKO (JP)
NAKAGAWA HIROAKI (JP)
SHIMASE AKIHIRO (JP)
NAKAJIMA RYOTA (JP)
IMAI KAZUMICHI (JP)
TAKEDA SHIZU (JP)

Application Number:

PCT/JP2014/065962

Publication Date:

December 31, 2014

Filing Date:

June 17, 2014

Export Citation:

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Assignee:

HITACHI HIGH TECH CORP (JP)

International Classes:

C12M1/34; C12Q1/06

Foreign References:

JP2004215585A	2004-08-05
JP2003520047A	2003-07-02
JP2014045663A	2014-03-17

Other References:

NAOKO SENDA ET AL.: "Hito Hyohi Saibo Sheet Baiyo Josei no Amino-San Bunseki", ANNUAL MEETING OF THE MOLECULAR BIOLOGY SOCIETY OF JAPAN PROGRAM YOSHISHU (WEB, vol. 36 TH, 20 November 2013 (2013-11-20)