Title:
METHOD FOR PREPARATION OF ACTIVE FORM OF LONG-ACTING INSULIN ANALOGUE CONJUGATE BY USING CLOSTRIPAIN
Document Type and Number:
WIPO Patent Application WO/2020/032423
Kind Code:
A1
Abstract:
The present invention relates to a method for preparation of an active form of a long-acting insulin analog conjugate, wherein the amino acid at position 22 on the insulin B chain is changed from arginine (Arg) to lysine (Lys) whereby the insulin can be converted to an active form without cleaving the insulin B chain even though reacting with clostripain. The preparation method according to the present invention surmounts the problem with a conventional method using trypsin in converting proinsulin to an active form that it is difficult to convert a long-acting insulin analog conjugate to an active form due to the cleavage of the albumin binding domain. Thus, the method of the present invention can be advantageously used for producing a long-acting therapeutic agent for diabetes.
Inventors:
AHN KYONG HOON (KR)
JEONG SEONKYEONG (KR)
YOON CHAEHA (KR)
JEONG SEONKYEONG (KR)
YOON CHAEHA (KR)
Application Number:
PCT/KR2019/008958
Publication Date:
February 13, 2020
Filing Date:
July 19, 2019
Export Citation:
Assignee:
DAE WOONG PHARMA (KR)
International Classes:
C07K14/62; A61K38/00; A61K47/64
Domestic Patent References:
| WO2014048977A1 | 2014-04-03 | |||
| WO2014048977A1 | 2014-04-03 |
Foreign References:
| KR20150138101A | 2015-12-09 | |||
| KR20150087130A | 2015-07-29 | |||
| US6841361B1 | 2005-01-11 | |||
| KR20017013921A | 2001-10-30 | |||
| KR20150058454A | 2015-05-28 |
Other References:
DUTT AROY, A. ET AL.: "Development of a long-acting insulin analog using albumin fusion technology", DIABETES, vol. 54, January 2005 (2005-01-01), pages 251 - 258, XP002499061, DOI: 10.2337/diabetes.54.1.251
See also references of EP 3835315A4
See also references of EP 3835315A4
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
Download PDF: