Title:
METHOD OF PRODUCING A RANDOMLY PRIMED CDNA SUITABLE FOR TARGETED CLONING
Document Type and Number:
WIPO Patent Application WO2000058458
Kind Code:
A3
Abstract:
The invention relates to a method of producing a randomly primed cDNA suitable for targeted cloning, which is based on the fact that during the synthesis of the first cDNA strand a single-strand oligonucleotide mixture is attached to an RNA or an (m)RNA mixture obtained from a sample. Said oligonucleotide mixture is characterized in that the 3' component comprises random sequences, preferably between 4 and 10 nucleotides in length, and the 5' component presents a recognition site for a restriction endonuclease when it is transferred to a double strand. The recognition site is preferably a recognition site for a restriction endonuclease which generates protruding ends.
Inventors:
DIEM CAROLINE DI
KORN BERNHARD (DE)
WIEMANN STEFAN (DE)
POUSTKA ANNEMARIE (DE)
KORN BERNHARD (DE)
WIEMANN STEFAN (DE)
POUSTKA ANNEMARIE (DE)
Application Number:
PCT/DE2000/000878
Publication Date:
April 19, 2001
Filing Date:
March 23, 2000
Export Citation:
Assignee:
DEUTSCHES KREBSFORSCH (DE)
GUENTHER STEFAN JENS HM (DE)
DIEM PAUL HM (DE)
DIEM FRIEDERIKE HF (DE)
KORN BERNHARD (DE)
WIEMANN STEFAN (DE)
POUSTKA ANNEMARIE (DE)
GUENTHER STEFAN JENS HM (DE)
DIEM PAUL HM (DE)
DIEM FRIEDERIKE HF (DE)
KORN BERNHARD (DE)
WIEMANN STEFAN (DE)
POUSTKA ANNEMARIE (DE)
International Classes:
C12N15/10; (IPC1-7): C12N15/11; C12N15/66
Foreign References:
| US5629179A | 1997-05-13 |
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