Title:
METHOD FOR RAPIDLY DETECTING ESTROGENIC AND ANDROGENIC COMPOUND, USING RECEPTOR PROTEIN EXPRESSED IN VITRO
Document Type and Number:
WIPO Patent Application WO/2023/204634
Kind Code:
A1
Abstract:
The present invention relates to a method for rapidly detecting an estrogenic and an androgenic compound, and the method for rapidly detecting an estrogenic and an androgenic compound, according to the present invention, is a method for detecting an interaction between estrogen or androgen receptors and a compound, through measurement of dimerization activity between the estrogen or androgen receptors mediated by the compound, by applying a bioluminescence resonance energy delivery outside cells, without using cells. Therefore, it is expected that the present invention can provide, more rapidly and easily compared to conventional estrogenic or androgenic compound detection methods, estrogenic or androgenic information of a compound mediated by an estrogen or androgen receptor.
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Inventors:
PARK YOOHEON (KR)
LEE SEOK-HEE (KR)
SEO HYEYOENG (KR)
LEE SEOK-HEE (KR)
SEO HYEYOENG (KR)
Application Number:
PCT/KR2023/005389
Publication Date:
October 26, 2023
Filing Date:
April 20, 2023
Export Citation:
Assignee:
UNIV DONGGUK IND ACAD COOP (KR)
International Classes:
G01N33/74; C07K14/72; G01N21/76; G01N33/53; G01N33/58
Foreign References:
| KR20220053768A | 2022-05-02 |
Other References:
KIM HYE MI, SEO HYEYEONG, PARK YOOHEON, LEE HEE-SEOK, LEE SEOK-HEE, KO KWANG SUK: "Development of a Human Estrogen Receptor Dimerization Assay for the Estrogenic Endocrine-Disrupting Chemicals Using Bioluminescence Resonance Energy Transfer", INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, vol. 18, no. 16, 23 August 2021 (2021-08-23), pages 8875, XP093101193, DOI: 10.3390/ijerph18168875
KYUNGYOUN HONG, HEE-SEOK LEE, SEOK-HEE LEE, KWANG SUK KO, MARTIN D’AGOSTINO, KYONG-SUP YOON, YOOHEON PARK: "P09-181 BRET (Bioluminescence Resonance Energy Transfer)-based androgen binding assay detecting androgenic chemical compounds in foods and related materials", 2019 KFN INTERNATIONAL SYMPOSIUM AND ANNUAL MEETING; OCTOBER 23-25, 2019, KOREAN SOCIETY OF FOOD AND NUTRITION SCIENCES, KOREA, 1 January 2019 (2019-01-01) - 25 October 2019 (2019-10-25), Korea, pages 435 - 436, XP009550337
HUIWON SEO, HEE-SEOK LEE, SEOK-HEE LEE: " Development of BRET based estrogen binding assay detecting estrogenic chemical compounds", 2020 KOREAN SOCIETY OF FOOD AND NUTRITION SCIENCE CONFERENCE PROCEEDINGS, INTERNATIONAL SYMPOSIUM AND ANNUAL MEETING, KOREAN SOCIETY OF FOOD AND NUTRITION SCIENCES, 1 October 2020 (2020-10-01), pages 469, XP009549683
POWELL, E. ; HUANG, S.X. ; XU, Y. ; RAJSKI, S.R. ; WANG, Y. ; PETERS, N. ; GUO, S. ; ERIC XU, H. ; HOFFMANN, F.M. ; SHEN, B. ; XU,: "Identification and characterization of a novel estrogenic ligand actinopolymorphol A", BIOCHEMICAL PHARMACOLOGY, ELSEVIER, US, vol. 80, no. 8, 15 October 2010 (2010-10-15), US , pages 1221 - 1229, XP027252719, ISSN: 0006-2952
LEE SEOK-HEE; HONG KYUNG YOUN; SEO HYEYEONG; LEE HEE-SEOK; PARK YOOHEON: "Mechanistic insight into human androgen receptor-mediated endocrine-disrupting potentials by a stable bioluminescence resonance energy transfer-based dimerization assay", CHEMICO-BIOLOGICAL INTERACTIONS., ELSEVIER SCIENCE IRLAND., IR, vol. 349, 11 September 2021 (2021-09-11), IR , XP086826824, ISSN: 0009-2797, DOI: 10.1016/j.cbi.2021.109655
KYUNGYOUN HONG, HEE-SEOK LEE, SEOK-HEE LEE, KWANG SUK KO, MARTIN D’AGOSTINO, KYONG-SUP YOON, YOOHEON PARK: "P09-181 BRET (Bioluminescence Resonance Energy Transfer)-based androgen binding assay detecting androgenic chemical compounds in foods and related materials", 2019 KFN INTERNATIONAL SYMPOSIUM AND ANNUAL MEETING; OCTOBER 23-25, 2019, KOREAN SOCIETY OF FOOD AND NUTRITION SCIENCES, KOREA, 1 January 2019 (2019-01-01) - 25 October 2019 (2019-10-25), Korea, pages 435 - 436, XP009550337
HUIWON SEO, HEE-SEOK LEE, SEOK-HEE LEE: " Development of BRET based estrogen binding assay detecting estrogenic chemical compounds", 2020 KOREAN SOCIETY OF FOOD AND NUTRITION SCIENCE CONFERENCE PROCEEDINGS, INTERNATIONAL SYMPOSIUM AND ANNUAL MEETING, KOREAN SOCIETY OF FOOD AND NUTRITION SCIENCES, 1 October 2020 (2020-10-01), pages 469, XP009549683
POWELL, E. ; HUANG, S.X. ; XU, Y. ; RAJSKI, S.R. ; WANG, Y. ; PETERS, N. ; GUO, S. ; ERIC XU, H. ; HOFFMANN, F.M. ; SHEN, B. ; XU,: "Identification and characterization of a novel estrogenic ligand actinopolymorphol A", BIOCHEMICAL PHARMACOLOGY, ELSEVIER, US, vol. 80, no. 8, 15 October 2010 (2010-10-15), US , pages 1221 - 1229, XP027252719, ISSN: 0006-2952
LEE SEOK-HEE; HONG KYUNG YOUN; SEO HYEYEONG; LEE HEE-SEOK; PARK YOOHEON: "Mechanistic insight into human androgen receptor-mediated endocrine-disrupting potentials by a stable bioluminescence resonance energy transfer-based dimerization assay", CHEMICO-BIOLOGICAL INTERACTIONS., ELSEVIER SCIENCE IRLAND., IR, vol. 349, 11 September 2021 (2021-09-11), IR , XP086826824, ISSN: 0009-2797, DOI: 10.1016/j.cbi.2021.109655
Attorney, Agent or Firm:
LEE, Myoung-Jin (KR)
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