Title:
METHOD FOR REFOLDING NEISSERIAL NSPA PROTEIN
Document Type and Number:
WIPO Patent Application WO2004020452
Kind Code:
A3
Abstract:
The present invention provides an isolated refolded NspA protein, and a method of preparing it.
Inventors:
BIEMANS RALPH (BE)
BOS MARTINE (NL)
DENOEL PHILIPPE (BE)
FERON CHRISTIANE (BE)
GORAJ KARINE (BE)
POOLMAN JAN (BE)
TOMMASSEN JAN (NL)
WEYNANTS VINCENT (BE)
BOS MARTINE (NL)
DENOEL PHILIPPE (BE)
FERON CHRISTIANE (BE)
GORAJ KARINE (BE)
POOLMAN JAN (BE)
TOMMASSEN JAN (NL)
WEYNANTS VINCENT (BE)
Application Number:
PCT/EP2003/010085
Publication Date:
August 19, 2004
Filing Date:
August 28, 2003
Export Citation:
Assignee:
GLAXOSMITHKLINE BIOLOG SA (BE)
UNIV UTRECHT (NL)
BIEMANS RALPH (BE)
BOS MARTINE (NL)
DENOEL PHILIPPE (BE)
FERON CHRISTIANE (BE)
GORAJ KARINE (BE)
POOLMAN JAN (BE)
TOMMASSEN JAN (NL)
WEYNANTS VINCENT (BE)
UNIV UTRECHT (NL)
BIEMANS RALPH (BE)
BOS MARTINE (NL)
DENOEL PHILIPPE (BE)
FERON CHRISTIANE (BE)
GORAJ KARINE (BE)
POOLMAN JAN (BE)
TOMMASSEN JAN (NL)
WEYNANTS VINCENT (BE)
International Classes:
A61K39/095; A61K39/102; C07K14/22; A61K39/00; (IPC1-7): C07K14/22; A61K39/095
Domestic Patent References:
| WO1996029412A1 | 1996-09-26 | |||
| WO2000071725A2 | 2000-11-30 | |||
| WO2001072337A1 | 2001-10-04 | |||
| WO2001009350A2 | 2001-02-08 |
Other References:
JANSEN C ET AL: "Biochemical and biophysical characterization of in vitro folded outer membrane porin PorA of Neisseria meningitidis", BIOCHIMICA ET BIOPHYSICA ACTA. BIOMEMBRANES, AMSTERDAM, NL, vol. 1464, no. 2, 5 April 2000 (2000-04-05), pages 284 - 298, XP004273195, ISSN: 0005-2736
KRAMER R A ET AL: "In vitro folding, purification and characterization of Escherichia coli outer membrane protease OmpT", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 267, no. 3, February 2000 (2000-02-01), pages 885 - 893, XP001189705, ISSN: 0014-2956
DEKKER NIEK ET AL: "In vitro folding of Escherichia coli outer-membrane phospholipase A", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 232, no. 1, 1995, pages 214 - 219, XP008029422, ISSN: 0014-2956
MOE G R ET AL: "Differences in surface expression of NspA among Neisseria meningitidis group B strains", INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY. WASHINGTON, US, vol. 67, no. 11, November 1999 (1999-11-01), pages 5664 - 5675, XP002266209, ISSN: 0019-9567
VANDEPUTTE-RUTTEN LUCY ET AL: "Crystal structure of neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 27, 4 July 2003 (2003-07-04), pages 24825 - 24830, XP002274972, ISSN: 0021-9258
KRAMER R A ET AL: "In vitro folding, purification and characterization of Escherichia coli outer membrane protease OmpT", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 267, no. 3, February 2000 (2000-02-01), pages 885 - 893, XP001189705, ISSN: 0014-2956
DEKKER NIEK ET AL: "In vitro folding of Escherichia coli outer-membrane phospholipase A", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 232, no. 1, 1995, pages 214 - 219, XP008029422, ISSN: 0014-2956
MOE G R ET AL: "Differences in surface expression of NspA among Neisseria meningitidis group B strains", INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY. WASHINGTON, US, vol. 67, no. 11, November 1999 (1999-11-01), pages 5664 - 5675, XP002266209, ISSN: 0019-9567
VANDEPUTTE-RUTTEN LUCY ET AL: "Crystal structure of neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 27, 4 July 2003 (2003-07-04), pages 24825 - 24830, XP002274972, ISSN: 0021-9258
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