Title:
METHOD FOR RENDERING TISSUE TRANSPARENT, REAGENT FOR RENDERING TISSUE TRANSPARENT, AND TISSUE OBSERVATION METHOD
Document Type and Number:
WIPO Patent Application WO/2014/115206
Kind Code:
A1
Abstract:
A method for rendering tissue transparent, said method including a step in which tissue is immersed in a water-soluble solvent including 2,2'-thiodiethanol, and glycerol and/or a non-ionic organic iodine compound, is provided as a technique with which, using a simple operation, and without using toxic or hazardous organic solvents, various organs can be rendered sufficiently transparent without inducing changes therein. In this method for rendering tissue transparent, it is preferable that a mixed solvent of 2,2'-thiodiethanol, glycerol, and a non-ionic organic iodine-compound aqueous solution be used for the water-soluble solvent.
Inventors:
ONODERA HIROSHI (JP)
Application Number:
PCT/JP2013/006811
Publication Date:
July 31, 2014
Filing Date:
November 20, 2013
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
International Classes:
C12Q1/00; G01N1/30; G01N1/28; G01N21/64; G02B21/34
Domestic Patent References:
| WO2011111876A1 | 2011-09-15 | |||
| WO2012161143A1 | 2012-11-29 | |||
| WO2012147965A1 | 2012-11-01 |
Foreign References:
| JP2007051957A | 2007-03-01 | |||
| US6472216B1 | 2002-10-29 | |||
| JPH11187900A | 1999-07-13 | |||
| JP2013522590A | 2013-06-13 |
Other References:
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APPLETON P.L. ET AL.: "Preparation of wholemount mouse intestine for high-resolution three-dimensional imaging using two-photon microscopy", J MICROSC, vol. 234, no. 2, May 2009 (2009-05-01), pages 196 - 204, XP055276873
PHILBERT S. TSAI: "Correlations of Neuronal and Microvascular Densities in Murine Cortex Revealed by Direct Counting and Colocalization of Nuclei and Vessels", THE JOURNAL OF NEUROSCIENCE, vol. 29, no. 46, 2009, pages 14553 - 14570, XP055182778
MARTIN B D ET AL.: "Hydroxylated secondary dopants for surface resistance enhancement in transparent poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) thin films", SYNTH MET, vol. 142, no. 1-3, 13 April 2004 (2004-04-13), pages 187 - 193, XP002357393
"Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury", NATURE MEDICINE, vol. 18, no. 1, 2011, pages 166 - 71
"Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain", NATURE NEUROSCIENCE, vol. 14, no. 11, 2011, pages 1481 - 1488
"Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the thy-1.2 promoter", PLOS ONE, vol. 4, no. 11, 2009, pages E7679
"Labeling and confocal imaging of neurons in thick invertebrate tissue samples", 2013, COLD SPRING HARBOR PROTOCOL
"2,2'-thiodiethanol: a new water soluble mounting medium for high resolution optical microscopy", MICROSCOPY RESEARCH AND TECHNIQUE, vol. 70, 2007, pages 1 - 9
P.T.GONZALEZ-BELLIDO ET AL.: "Labeling amd confocal imaging of neurons in thick inverterbrate tissue samples", COLD SPRING HARB PROTOC, September 2012 (2012-09-01), pages 970 - 983, XP055276872
APPLETON P.L. ET AL.: "Preparation of wholemount mouse intestine for high-resolution three-dimensional imaging using two-photon microscopy", J MICROSC, vol. 234, no. 2, May 2009 (2009-05-01), pages 196 - 204, XP055276873
PHILBERT S. TSAI: "Correlations of Neuronal and Microvascular Densities in Murine Cortex Revealed by Direct Counting and Colocalization of Nuclei and Vessels", THE JOURNAL OF NEUROSCIENCE, vol. 29, no. 46, 2009, pages 14553 - 14570, XP055182778
MARTIN B D ET AL.: "Hydroxylated secondary dopants for surface resistance enhancement in transparent poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) thin films", SYNTH MET, vol. 142, no. 1-3, 13 April 2004 (2004-04-13), pages 187 - 193, XP002357393
"Three-dimensional imaging of the unsectioned adult spinal cord to assess axon regeneration and glial responses after injury", NATURE MEDICINE, vol. 18, no. 1, 2011, pages 166 - 71
"Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain", NATURE NEUROSCIENCE, vol. 14, no. 11, 2011, pages 1481 - 1488
"Visual properties of transgenic rats harboring the channelrhodopsin-2 gene regulated by the thy-1.2 promoter", PLOS ONE, vol. 4, no. 11, 2009, pages E7679
"Labeling and confocal imaging of neurons in thick invertebrate tissue samples", 2013, COLD SPRING HARBOR PROTOCOL
"2,2'-thiodiethanol: a new water soluble mounting medium for high resolution optical microscopy", MICROSCOPY RESEARCH AND TECHNIQUE, vol. 70, 2007, pages 1 - 9
Attorney, Agent or Firm:
OHNO, Seiji et al. (JP)
Seiji Ono (JP)
Seiji Ono (JP)
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