Title:
METHOD FOR USING REF PROTEIN AS A TARGETED RECA-DEPENDENT NUCLEASE
Document Type and Number:
WIPO Patent Application WO/2012/021803
Kind Code:
A8
Abstract:
Kits and a method for cleaving double-stranded DNA using Ref and RecA protein and variants thereof at a site having a DNA sequence homologous to the sequence on a single- stranded DNA targeting fragment are disclosed.
Inventors:
COX MICHAEL M (US)
GRUENIG MARIELLE C (DE)
KECK JAMES L (US)
GRUENIG MARIELLE C (DE)
KECK JAMES L (US)
Application Number:
PCT/US2011/047584
Publication Date:
October 04, 2012
Filing Date:
August 12, 2011
Export Citation:
Assignee:
WISCONSIN ALUMNI RES FOUND (US)
COX MICHAEL M (US)
GRUENIG MARIELLE C (DE)
KECK JAMES L (US)
COX MICHAEL M (US)
GRUENIG MARIELLE C (DE)
KECK JAMES L (US)
International Classes:
C12N9/22; C07K14/01; C07K14/245; C12N9/00; C12N15/34; C12N15/52; C12N15/55; C12N15/66; C12Q1/68; C12N15/113; C30B29/58
Attorney, Agent or Firm:
HEIDMANN, Keith, H. (411 E. Wisconsin AvenueMilwaukee, WI, US)
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