Title:
METHODS FOR THE PREPARATION OF A FINE CHEMICAL BY FERMENTATION
Document Type and Number:
WIPO Patent Application WO2005059154
Kind Code:
A3
Abstract:
The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., lactate dehydrogenase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of the expression of lactate dehydrogenase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.
More Like This:
Inventors:
ZELDER OSKAR (DE)
KLOPPROGGE CORINNA (DE)
SCHROEDER HARTWIG (DE)
HAEFNER STEFAN (DE)
KROEGER BURKHARD (DE)
KIEFER PATRICK (DE)
HEINZLE ELMAR (DE)
WITTMANN CHRISTOPH (DE)
KLOPPROGGE CORINNA (DE)
SCHROEDER HARTWIG (DE)
HAEFNER STEFAN (DE)
KROEGER BURKHARD (DE)
KIEFER PATRICK (DE)
HEINZLE ELMAR (DE)
WITTMANN CHRISTOPH (DE)
Application Number:
PCT/IB2004/004426
Publication Date:
October 13, 2005
Filing Date:
December 17, 2004
Export Citation:
Assignee:
BASF AG (DE)
ZELDER OSKAR (DE)
KLOPPROGGE CORINNA (DE)
SCHROEDER HARTWIG (DE)
HAEFNER STEFAN (DE)
KROEGER BURKHARD (DE)
KIEFER PATRICK (DE)
HEINZLE ELMAR (DE)
WITTMANN CHRISTOPH (DE)
ZELDER OSKAR (DE)
KLOPPROGGE CORINNA (DE)
SCHROEDER HARTWIG (DE)
HAEFNER STEFAN (DE)
KROEGER BURKHARD (DE)
KIEFER PATRICK (DE)
HEINZLE ELMAR (DE)
WITTMANN CHRISTOPH (DE)
International Classes:
C12N9/04; C12P13/08; (IPC1-7): C12P13/08; C12N1/21; C12N9/04; C12N15/53
Domestic Patent References:
| WO2001007626A2 | 2001-02-01 | |||
| WO2001000844A2 | 2001-01-04 | |||
| WO2003014330A2 | 2003-02-20 |
Foreign References:
| US20020055152A1 | 2002-05-09 |
Other References:
MARX ET AL: "Response of central metabolism in Corynebacterium glutamicum to the use of an NADH-dependent glutamate dehydrogenase", METABOLIC ENGINEERING, ACADEMIC PRESS,, US, vol. 1, 1999, pages 35 - 48, XP002154493, ISSN: 1096-7176
PARK S M ET AL: "METABOLIC AND PHYSIOLOGICAL STUDIES OF CORYNEBACTERIUM GLUTAMICUM MUTANTS", BIOTECHNOLOGY AND BIOENGINEERING, INTERSCIENCE PUBLISHERS, LONDON, GB, vol. 55, no. 6, 1997, pages 864 - 879, XP000999162, ISSN: 0006-3592
PATENT ABSTRACTS OF JAPAN vol. 1999, no. 13 30 November 1999 (1999-11-30)
HOLS P ET AL: "Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, no. 6, June 1999 (1999-06-01), pages 588 - 592, XP002225554, ISSN: 1087-0156
GOKARN R R ET AL: "The physiological effects and metabolic alterations caused by the expression of Rhizobium etli pyruvate carboxylase in Escherichia coli.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. JUL 2001, vol. 56, no. 1-2, July 2001 (2001-07-01), pages 188 - 195, XP002287974, ISSN: 0175-7598
DOMINGUEZ H ET AL: "CARBON-FLUX DISTRIBUTION IN THE CENTRAL METABOLIC PATHWAYS OF CORYNEBACTERIUM GLUTAMICUM DURING GROWTH ON FRUCTOSE", EUROPEAN JOURNAL OF BIOCHEMISTRY, BERLIN, DE, vol. 254, no. 1, 15 May 1998 (1998-05-15), pages 96 - 102, XP000960354, ISSN: 0014-2956
BOTT MICHAEL ET AL: "The respiratory chain of Corynebacterium glutamicum.", JOURNAL OF BIOTECHNOLOGY. 4 SEP 2003, vol. 104, no. 1-3, 4 September 2003 (2003-09-04), pages 129 - 153, XP002288114, ISSN: 0168-1656
KIEFER P ET AL: "Influence of glucose, fructose and sucrose as carbon sources on kinetics and stoichiometry of lysine production by Corynebacterium glutamicum.", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY. JUN 2002, vol. 28, no. 6, June 2002 (2002-06-01), pages 338 - 343, XP002287975, ISSN: 1367-5435
SAHM H ET AL: "PATHWAY ANALYSIS AND METABOLIC ENGINEERING IN CORYNEBACTERIUM GLUTAMICUM", BIOLOGICAL CHEMISTRY, XX, XX, vol. 381, no. 9/10, September 2000 (2000-09-01), pages 899 - 910, XP000979510, ISSN: 1431-6730
PEQUIGNOT C ET AL: "Effect of oxygen transfer and carbon source composition on the growth of Corynebacterium melassecola", BIOPROCESS ENGINEERING 1995 GERMANY, vol. 13, no. 4, 1995, pages 197 - 204, XP008032761, ISSN: 0178-515X
MARX ACHIM ET AL: "Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing", BIOTECHNOLOGY AND BIOENGINEERING, vol. 49, no. 2, 1996, pages 111 - 129, XP002287973, ISSN: 0006-3592
KIEFER PATRICK ET AL: "Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY. JAN 2004, vol. 70, no. 1, January 2004 (2004-01-01), pages 229 - 239, XP002287976, ISSN: 0099-2240
PARK S M ET AL: "METABOLIC AND PHYSIOLOGICAL STUDIES OF CORYNEBACTERIUM GLUTAMICUM MUTANTS", BIOTECHNOLOGY AND BIOENGINEERING, INTERSCIENCE PUBLISHERS, LONDON, GB, vol. 55, no. 6, 1997, pages 864 - 879, XP000999162, ISSN: 0006-3592
PATENT ABSTRACTS OF JAPAN vol. 1999, no. 13 30 November 1999 (1999-11-30)
HOLS P ET AL: "Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, no. 6, June 1999 (1999-06-01), pages 588 - 592, XP002225554, ISSN: 1087-0156
GOKARN R R ET AL: "The physiological effects and metabolic alterations caused by the expression of Rhizobium etli pyruvate carboxylase in Escherichia coli.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. JUL 2001, vol. 56, no. 1-2, July 2001 (2001-07-01), pages 188 - 195, XP002287974, ISSN: 0175-7598
DOMINGUEZ H ET AL: "CARBON-FLUX DISTRIBUTION IN THE CENTRAL METABOLIC PATHWAYS OF CORYNEBACTERIUM GLUTAMICUM DURING GROWTH ON FRUCTOSE", EUROPEAN JOURNAL OF BIOCHEMISTRY, BERLIN, DE, vol. 254, no. 1, 15 May 1998 (1998-05-15), pages 96 - 102, XP000960354, ISSN: 0014-2956
BOTT MICHAEL ET AL: "The respiratory chain of Corynebacterium glutamicum.", JOURNAL OF BIOTECHNOLOGY. 4 SEP 2003, vol. 104, no. 1-3, 4 September 2003 (2003-09-04), pages 129 - 153, XP002288114, ISSN: 0168-1656
KIEFER P ET AL: "Influence of glucose, fructose and sucrose as carbon sources on kinetics and stoichiometry of lysine production by Corynebacterium glutamicum.", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY. JUN 2002, vol. 28, no. 6, June 2002 (2002-06-01), pages 338 - 343, XP002287975, ISSN: 1367-5435
SAHM H ET AL: "PATHWAY ANALYSIS AND METABOLIC ENGINEERING IN CORYNEBACTERIUM GLUTAMICUM", BIOLOGICAL CHEMISTRY, XX, XX, vol. 381, no. 9/10, September 2000 (2000-09-01), pages 899 - 910, XP000979510, ISSN: 1431-6730
PEQUIGNOT C ET AL: "Effect of oxygen transfer and carbon source composition on the growth of Corynebacterium melassecola", BIOPROCESS ENGINEERING 1995 GERMANY, vol. 13, no. 4, 1995, pages 197 - 204, XP008032761, ISSN: 0178-515X
MARX ACHIM ET AL: "Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing", BIOTECHNOLOGY AND BIOENGINEERING, vol. 49, no. 2, 1996, pages 111 - 129, XP002287973, ISSN: 0006-3592
KIEFER PATRICK ET AL: "Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY. JAN 2004, vol. 70, no. 1, January 2004 (2004-01-01), pages 229 - 239, XP002287976, ISSN: 0099-2240
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