Title:
MULTI-REPORTER GENE MODEL FOR TOXICOLOGICAL SCREENING
Document Type and Number:
WIPO Patent Application WO2004011676
Kind Code:
A3
Abstract:
A system for the detection of gene activation events is provided which comprises a nucleic acid construct encoding a protein of the lipocalin protein family and a peptide tag in which the expression of the construct in a cell or in the cells of a transgenic animal demonstrates the activation of a gene or genes of interest, in which the protein expressed is secreted from the cell and in which detection of the peptide tag indicates expression of the construct.
Inventors:
WHITELAW CHRISTOPHER BRUCE ALE (GB)
CLARK ANTHONY JOHN (GB)
WOLF CHARLES ROLAND (GB)
CLARK ANTHONY JOHN (GB)
WOLF CHARLES ROLAND (GB)
Application Number:
PCT/GB2003/003192
Publication Date:
May 06, 2004
Filing Date:
July 25, 2003
Export Citation:
Assignee:
ROSLIN INST EDINBURGH (GB)
CXR BIOSCIENCES LTD (GB)
WHITELAW CHRISTOPHER BRUCE ALE (GB)
CLARK ANTHONY JOHN (GB)
WOLF CHARLES ROLAND (GB)
CXR BIOSCIENCES LTD (GB)
WHITELAW CHRISTOPHER BRUCE ALE (GB)
CLARK ANTHONY JOHN (GB)
WOLF CHARLES ROLAND (GB)
International Classes:
A01K67/027; C07K14/47; C12N15/62; C12N15/85; G01N33/50; (IPC1-7): C12N15/62; C07K14/47; C12Q1/68; G01N33/68
Domestic Patent References:
| WO2002053701A2 | 2002-07-11 | |||
| WO2000075308A1 | 2000-12-14 | |||
| WO1997007132A1 | 1997-02-27 | |||
| WO1998030715A1 | 1998-07-16 | |||
| WO2002001949A2 | 2002-01-10 | |||
| WO1999037142A1 | 1999-07-29 | |||
| WO2002031151A2 | 2002-04-18 |
Foreign References:
| US6114123A | 2000-09-05 | |||
| US5948677A | 1999-09-07 | |||
| EP1130086A1 | 2001-09-05 | |||
| EP1111387A1 | 2001-06-27 |
Other References:
MCCLENAGHAN M ET AL: "Insertion of a casein kinase recognition sequence induces phosphorylation of ovine beta-lactoglobulin in transgenic mice", PROTEIN ENGINEERING, vol. 12, no. 3, March 1999 (1999-03-01), pages 259 - 264, XP002259482, ISSN: 0269-2139
SCHLEHUBER STEFFEN ET AL: "A novel type of receptor protein, based on the lipocalin scaffold, with specificity for digoxigenin", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 297, no. 5, 14 April 2000 (2000-04-14), pages 1105 - 1120, XP002150338, ISSN: 0022-2836
SUI DEXIN; WILSON JOHN E: "Interaction of insulin-like growth factor binding protein-4, Miz-1, leptin , lipocalin-typprostaglandin D synthase, and granulin precursor with the N-terminal half of Type III -hexokinase", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 382, no. 2, 15 October 2000 (2000-10-15), pages 262 - 274, XP001172430
SUNDARAM M ET AL: "Expression, characterization and engineered specificity of rat epididymal retinoic-acid binding protein", BIOCHEMICAL JOURNAL, PORTLAND PRESS, LONDON, GB, vol. 334, no. 1, 15 August 1998 (1998-08-15), pages 155 - 160, XP002095127, ISSN: 0264-6021
SCHMIDT A M ET AL: "A ZN(II)-BINDING SITE ENGINEERED INTO RETINOL-BINDING PROTEIN EXHIBITS METAL-ION SPECIFICITY AND ALLOWS HIGHLY EFFICIENT AFFINITY PURIFICATION WITH A NEWLY DESIGNED METAL LIGAND", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 3, no. 8, August 1996 (1996-08-01), pages 645 - 653, XP001117565, ISSN: 1074-5521
CROSSETT, B. ET AL.: "Transfer of a uterine lipocalin from the endometrium of the mare to the developing equine conceptus", BIOLOGY OF REPRODUCTION, vol. 59, 1998, pages 483 - 490, XP002259483
SCHLEHUBER STEFFEN ET AL: "A novel type of receptor protein, based on the lipocalin scaffold, with specificity for digoxigenin", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 297, no. 5, 14 April 2000 (2000-04-14), pages 1105 - 1120, XP002150338, ISSN: 0022-2836
SUI DEXIN; WILSON JOHN E: "Interaction of insulin-like growth factor binding protein-4, Miz-1, leptin , lipocalin-typprostaglandin D synthase, and granulin precursor with the N-terminal half of Type III -hexokinase", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 382, no. 2, 15 October 2000 (2000-10-15), pages 262 - 274, XP001172430
SUNDARAM M ET AL: "Expression, characterization and engineered specificity of rat epididymal retinoic-acid binding protein", BIOCHEMICAL JOURNAL, PORTLAND PRESS, LONDON, GB, vol. 334, no. 1, 15 August 1998 (1998-08-15), pages 155 - 160, XP002095127, ISSN: 0264-6021
SCHMIDT A M ET AL: "A ZN(II)-BINDING SITE ENGINEERED INTO RETINOL-BINDING PROTEIN EXHIBITS METAL-ION SPECIFICITY AND ALLOWS HIGHLY EFFICIENT AFFINITY PURIFICATION WITH A NEWLY DESIGNED METAL LIGAND", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 3, no. 8, August 1996 (1996-08-01), pages 645 - 653, XP001117565, ISSN: 1074-5521
CROSSETT, B. ET AL.: "Transfer of a uterine lipocalin from the endometrium of the mare to the developing equine conceptus", BIOLOGY OF REPRODUCTION, vol. 59, 1998, pages 483 - 490, XP002259483
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