Title:
MUTEINS OF HUMAN NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN AND RELATED PROTEINS
Document Type and Number:
WIPO Patent Application WO2003029463
Kind Code:
A3
Abstract:
Disclosed is a method for generating a mutein of a protein selected from the group consisting of human neutrophil gelatinase-associated lipocalin (hNGAL), rat alpha 2- microglobulin-related protein (A2m) and mouse 24p3/uterocalin (24p3), said mutein having detectable affinity to a given target. The method comprises the step of subjecting the protein to mutagenesis at one or more of the sequence positions which correspond to the sequence positions 33 to 54, 66 to 83, 94 to 106, and 123 to 136 of hNGAL, resulting in one or more mutein(s) of the protein. Also disclosed are muteins obtainable by this method.
Inventors:
SKERRA ARNE (DE)
SCHLEHUBER STEFFEN (DE)
SCHLEHUBER STEFFEN (DE)
Application Number:
PCT/EP2002/010490
Publication Date:
November 27, 2003
Filing Date:
September 18, 2002
Export Citation:
Assignee:
PIERIS PROTEOLAB AG (DE)
SKERRA ARNE (DE)
SCHLEHUBER STEFFEN (DE)
SKERRA ARNE (DE)
SCHLEHUBER STEFFEN (DE)
International Classes:
A61K38/00; A61K38/17; A61P43/00; C07K14/47; C07K19/00; C12N15/09; C12N15/12; C12N15/62; C12P21/02; G01N33/68; (IPC1-7): C12N15/12; C07K14/47; C12N15/62; A61K38/17; G01N33/68
Domestic Patent References:
| WO1999016873A1 | 1999-04-08 | |||
| WO2000075308A1 | 2000-12-14 | |||
| WO1996023879A1 | 1996-08-08 |
Other References:
BESTE G. ET AL.: "Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 96, no. 5, 2 March 1999 (1999-03-02), pages 1898 - 1903, XP002150337, ISSN: 0027-8424
STOESZ S.P. AND GOULD M.N.: "Overexpression of neu-related lipocalin (NRL) in neu-initiated but not ras or chemically initiated rat mammary carcinomas", ONCOGENE, vol. 11, 1995, pages 2233 - 2241, XP008015618
CHAN Y.-L. ET AL.: "The primary structure of rat alpha 2mu globulin-related protein", NUCLEIC ACIDS RESEARCH, vol. 16, no. 23, 1988, pages 11368, XP001148818
BUNDGAARD J.R. ET AL.: "Molecular cloning and expression of a cDNA encoding NGAL: a Lipocalin expressed in human neutrophils", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 202, no. 3, 15 August 1994 (1994-08-15), pages 1468 - 1475, XP002036694, ISSN: 0006-291X
PAINE K. AND FLOWER D.R.: "The lipocalin website", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1482, no. 1-2, 18 October 2000 (2000-10-18), pages 351 - 352, XP004279087, ISSN: 0167-4838
SKERRA A.: "Lipocalins as a scaffold", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1482, no. 1-2, 18 October 2000 (2000-10-18), pages 337 - 350, XP004279086, ISSN: 0167-4838
SCHLEHUBER S. ET AL.: "A novel type of receptor protein, based on the lipocalin scaffold, with specificity for digoxigenin", JOURNAL OF MOLECULAR BIOLOGY, vol. 297, no. 5, 14 April 2000 (2000-04-14), pages 1105 - 1120, XP002150338, ISSN: 0022-2836
STOESZ S.P. AND GOULD M.N.: "Overexpression of neu-related lipocalin (NRL) in neu-initiated but not ras or chemically initiated rat mammary carcinomas", ONCOGENE, vol. 11, 1995, pages 2233 - 2241, XP008015618
CHAN Y.-L. ET AL.: "The primary structure of rat alpha 2mu globulin-related protein", NUCLEIC ACIDS RESEARCH, vol. 16, no. 23, 1988, pages 11368, XP001148818
BUNDGAARD J.R. ET AL.: "Molecular cloning and expression of a cDNA encoding NGAL: a Lipocalin expressed in human neutrophils", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 202, no. 3, 15 August 1994 (1994-08-15), pages 1468 - 1475, XP002036694, ISSN: 0006-291X
PAINE K. AND FLOWER D.R.: "The lipocalin website", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1482, no. 1-2, 18 October 2000 (2000-10-18), pages 351 - 352, XP004279087, ISSN: 0167-4838
SKERRA A.: "Lipocalins as a scaffold", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1482, no. 1-2, 18 October 2000 (2000-10-18), pages 337 - 350, XP004279086, ISSN: 0167-4838
SCHLEHUBER S. ET AL.: "A novel type of receptor protein, based on the lipocalin scaffold, with specificity for digoxigenin", JOURNAL OF MOLECULAR BIOLOGY, vol. 297, no. 5, 14 April 2000 (2000-04-14), pages 1105 - 1120, XP002150338, ISSN: 0022-2836
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