Title:
NUCLEIC ACID CONSTRUCT CONTAINING FULL-LENGTH GENOME OF HUMAN HEPATITIS C VIRUS, RECOMBINANT FULL-LENGTH VIRUS GENOME REPLICATIVE CELL HAVING THE NUCLEIC ACID CONSTRUCT TRANSFERRED THEREINTO AND METHOD OF CONSTRUCTING HEPATITIS C VIRAL PARTICLES
Document Type and Number:
WIPO Patent Application WO/2005/080575
Kind Code:
A1
Abstract:
It is intended to provide a method of efficiently replicating RNA containing the full-length genome sequence of HCV; a method of preparing viral particles of HCV containing full-length HCV replicon RNA or full-length HCV genome RNA by using a cell culture system; a method of producing hepatitis C viral particles which comprises culturing cells having replicon RNA comprising the full-length RNA sequence of hepatitis C virus of the genotype 2a and a base sequence containing at least one selection marker and/or at least one reporter gene and at least one IRES sequence or the full-length genome RNA of hepatitis C virus of the genotype 2a to thereby allowing the production of viral particles in the culture medium; a hepatitis C vaccine; and an antibody against hepatitis C viral particles.
Inventors:
WAKITA TAKAJI (JP)
KATO TAKANOBU (JP)
DATE TOMOKO (JP)
MIYAMOTO MICHIKO (JP)
TANABE JUN-ICHI (JP)
SONE SABURO (JP)
KATO TAKANOBU (JP)
DATE TOMOKO (JP)
MIYAMOTO MICHIKO (JP)
TANABE JUN-ICHI (JP)
SONE SABURO (JP)
Application Number:
PCT/JP2005/003232
Publication Date:
September 01, 2005
Filing Date:
February 21, 2005
Export Citation:
Assignee:
TOKYO METROPOLITAN ORG MED RES (JP)
TORAY INDUSTRIES (JP)
WAKITA TAKAJI (JP)
KATO TAKANOBU (JP)
DATE TOMOKO (JP)
MIYAMOTO MICHIKO (JP)
TANABE JUN-ICHI (JP)
SONE SABURO (JP)
TORAY INDUSTRIES (JP)
WAKITA TAKAJI (JP)
KATO TAKANOBU (JP)
DATE TOMOKO (JP)
MIYAMOTO MICHIKO (JP)
TANABE JUN-ICHI (JP)
SONE SABURO (JP)
International Classes:
A61K35/76; A61K38/00; A61K39/29; A61K48/00; A61P1/16; A61P31/12; A61P37/04; C07K16/08; C07K16/10; C12N1/15; C12N1/19; C12N1/21; C12N5/00; C12N7/00; C12N7/01; C12N7/02; C12N15/09; C12N15/63; C12N15/86; C12Q1/02; C12Q1/68; G01N33/15; G01N33/50; G01N33/53; G01N33/567; G01N33/576; (IPC1-7): C12N15/63; C12N1/15; C12N1/19; C12N1/21; C12N5/00; C12N7/01; C12Q1/02; C12Q1/68; G01N33/15; G01N33/50; G01N33/53; G01N33/567; C07K16/08; A61K35/76; A61P37/04
Foreign References:
| JP2002171978A | 2002-06-18 |
Other References:
LECHMANN M. ET AL: "Hepatitis C virus-like particles induce virus-specific humoral and cellular immune responses in mice.", HEPATOLOGY., vol. 34, no. 2, August 2001 (2001-08-01), pages 417 - 423, XP008072763
LIM S.P. ET AL: "Inductible system in human hepatoma cell lines for hepatitis C virus production.", VIROLOGY., vol. 303, 2002, pages 79 - 99, XP008072779
IKEDA M. ET AL: "Selectable subgenomic and genome-length dicistronic RNAs derived from an infectious molecular clone of the HCV-N strain of hapatitis C virus replicate efficiently in cultured Huh7 cells.", J.VIROL., vol. 76, no. 6, March 2002 (2002-03-01), pages 2997 - 3006, XP002967510
See also references of EP 1721985A4
LIM S.P. ET AL: "Inductible system in human hepatoma cell lines for hepatitis C virus production.", VIROLOGY., vol. 303, 2002, pages 79 - 99, XP008072779
IKEDA M. ET AL: "Selectable subgenomic and genome-length dicistronic RNAs derived from an infectious molecular clone of the HCV-N strain of hapatitis C virus replicate efficiently in cultured Huh7 cells.", J.VIROL., vol. 76, no. 6, March 2002 (2002-03-01), pages 2997 - 3006, XP002967510
See also references of EP 1721985A4
Attorney, Agent or Firm:
Hiraki, Yusuke (3-20 Toranomon 4-chome, Minato-k, Tokyo 01, JP)
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