Title:
PHOTOCLEAVABLE ISOTOPE-CODED AFFINITY TAGS
Document Type and Number:
WIPO Patent Application WO2005037210
Kind Code:
A3
Abstract:
This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as proteins and protein fragments from biological samples from in vivo and in vitro sources. Agents comprise a detectable group bound to a photoreactive group. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals.
More Like This:
Inventors:
OLEJNIK JERZY (US)
ROTHSCHILD KENNETH J (US)
ROTHSCHILD KENNETH J (US)
Application Number:
PCT/US2004/033819
Publication Date:
March 02, 2006
Filing Date:
October 14, 2004
Export Citation:
Assignee:
AMBERGEN INC (US)
OLEJNIK JERZY (US)
ROTHSCHILD KENNETH J (US)
OLEJNIK JERZY (US)
ROTHSCHILD KENNETH J (US)
International Classes:
C07C233/56; C07D495/04; C07K14/00; G01N33/532; A61K; (IPC1-7): C07D235/02
Foreign References:
| US6852544B2 | 2005-02-08 | |||
| US5219764A | 1993-06-15 |
Other References:
TRESTER-ZEDLITZ M. ET AL: "A Modular Cross-Linking Approach for Exploring Protein Interactions", J. AM. CHEM. SOC., vol. 125, no. 9, 5 March 2003 (2003-03-05), pages 2416 - 2425, XP002994767
PANDORI M.W. ET AL: "Photochemical Control of the Infectivity of Adenoviral Vectors using a Novel Photocleavable Biotinylation Reagent", CHEMISTRY & BIOLOGY, vol. 9, May 2002 (2002-05-01), pages 567 - 573, XP002978764
PANDORI M.W. ET AL: "Photochemical Control of the Infectivity of Adenoviral Vectors using a Novel Photocleavable Biotinylation Reagent", CHEMISTRY & BIOLOGY, vol. 9, May 2002 (2002-05-01), pages 567 - 573, XP002978764
Download PDF: