Title:
PRODUCING BUTYL BUTYRATE FROM LIGNOCELLULOSIC BIOMASS THROUGH ESCHERICHIA COLI-CLOSTRIDIUM CO-CULTURE
Document Type and Number:
WIPO Patent Application WO/2022/271104
Kind Code:
A3
Abstract:
The present invention relates to compositions comprising a genetically engineered Escherichia coli and an acetone-butanol-ethanol (ABE)-producing Clostridium and methods of their use to provide valuable chemicals, such as butyl butyrate or butyl acetate from lignocellulosic biomass. More particularly said genetically engineered E. coli strain has been transformed by at least one polynucleotide molecule comprising a CoA transferase (CTF) gene and an alcohol acyltransferase (ATF) gene operably linked to at least one promoter.
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Inventors:
ZHOU KANG (SG)
YANG KUN-LIN (SG)
CUI YONGHAO (SG)
HE JIANZHONG (SG)
YANG KUN-LIN (SG)
CUI YONGHAO (SG)
HE JIANZHONG (SG)
Application Number:
PCT/SG2022/050431
Publication Date:
March 09, 2023
Filing Date:
June 24, 2022
Export Citation:
Assignee:
NAT UNIV SINGAPORE (SG)
International Classes:
C12P7/62; C12N9/10; C12N15/70; C12R1/145; C12R1/19
Other References:
SINUMVAYO JEAN PAUL, LI YIN, ZHANG YANPING: "Microbial production of butyl butyrate: from single strain to cognate consortium", BIORESOURCES AND BIOPROCESSING, vol. 8, no. 1, 1 December 2021 (2021-12-01), pages 50 - 11, XP093044488, ISSN: 2197-4365, DOI: 10.1186/s40643-021-00403-4
FENG JUN, ZHANG JIE, MA YUECHAO, FENG YIMING, WANG SHANGJUN, GUO NA, WANG HAIJIAO, WANG PIXIANG, JIMÉNEZ-BONILLA PABLO, GU YANYAN,: "Renewable fatty acid ester production in Clostridium", NATURE COMMUNICATIONS, NATURE PUBLISHING GROUP, UK, vol. 12, no. 1, 16 July 2021 (2021-07-16), UK, pages 4368 - 13, XP093044489, ISSN: 2041-1723, DOI: 10.1038/s41467-021-24038-3
CUI YONGHAO; HE JIANZHONG; YANG KUN-LIN; ZHOU KANG: "Production of isopropyl and butyl esters bymono-culture and co-culture", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, BASINGSTOKE, GB, vol. 47, no. 6-7, 16 May 2020 (2020-05-16), GB , pages 543 - 550, XP037200441, ISSN: 1367-5435, DOI: 10.1007/s10295-020-02279-3
NOH HYEON JI; WOO JI EUN; LEE SANG YUP; JANG YU-SIN: "Metabolic engineering ofClostridium acetobutylicumfor the production of butyl butyrate", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 102, no. 19, 3 August 2018 (2018-08-03), Berlin/Heidelberg, pages 8319 - 8327, XP036591463, ISSN: 0175-7598, DOI: 10.1007/s00253-018-9267-z
NOH H.J. ET AL.: "Microbial production of butyl butyrate, a flavor and fragrance compound", APPL MICROBIOL BIOTECHNOL, vol. 103, no. 5, 18 January 2019 (2019-01-18), pages 2079 - 2086, XP036727174, [retrieved on 20230130], DOI: 10.1007/S00253-018-09603-Z
FENG JUN, ZHANG JIE, MA YUECHAO, FENG YIMING, WANG SHANGJUN, GUO NA, WANG HAIJIAO, WANG PIXIANG, JIMÉNEZ-BONILLA PABLO, GU YANYAN,: "Renewable fatty acid ester production in Clostridium", NATURE COMMUNICATIONS, NATURE PUBLISHING GROUP, UK, vol. 12, no. 1, 16 July 2021 (2021-07-16), UK, pages 4368 - 13, XP093044489, ISSN: 2041-1723, DOI: 10.1038/s41467-021-24038-3
CUI YONGHAO; HE JIANZHONG; YANG KUN-LIN; ZHOU KANG: "Production of isopropyl and butyl esters bymono-culture and co-culture", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, BASINGSTOKE, GB, vol. 47, no. 6-7, 16 May 2020 (2020-05-16), GB , pages 543 - 550, XP037200441, ISSN: 1367-5435, DOI: 10.1007/s10295-020-02279-3
NOH HYEON JI; WOO JI EUN; LEE SANG YUP; JANG YU-SIN: "Metabolic engineering ofClostridium acetobutylicumfor the production of butyl butyrate", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 102, no. 19, 3 August 2018 (2018-08-03), Berlin/Heidelberg, pages 8319 - 8327, XP036591463, ISSN: 0175-7598, DOI: 10.1007/s00253-018-9267-z
NOH H.J. ET AL.: "Microbial production of butyl butyrate, a flavor and fragrance compound", APPL MICROBIOL BIOTECHNOL, vol. 103, no. 5, 18 January 2019 (2019-01-18), pages 2079 - 2086, XP036727174, [retrieved on 20230130], DOI: 10.1007/S00253-018-09603-Z
Attorney, Agent or Firm:
DOWSING, Bruce, John (SG)
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