Title:
REAGENT COMPOSITION FOR DETECTING β-LACTAMASE, DETECTION KIT AND DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2004/040008
Kind Code:
A1
Abstract:
It is intended to provide a reagent composition for detecting β-lactamase which contains 3-[2,4-dinitrostyryl]-7-(2-thienylacetamido)-3-cephem-4-carboxylic acid or 7-[2-(2-aminothiazol-4-yl)-2-(1-carboxy-1-methylethoxyimino)acetamido]-3-(2,4-dinitrostyryl)-3-cephem-4-carboxylic acid as a substrate for detecting β-lactamase together with at least one β-lactamase inhibitor selected from among clavulanic acid, aztreonam, ethylenediaminetetraacetic acid and cloxacillin and by which β-lactamase can be quickly and easily identified at a high sensitivity. Moreover, a detection kit containing the above detection reagent composition and a method of detecting β-lactamase comprising contacting a liquid specimen containing the subject to be analyzed with the above composition are provided.
Inventors:
MURATA ATSUSHI (JP)
Application Number:
PCT/JP2003/013835
Publication Date:
May 13, 2004
Filing Date:
October 29, 2003
Export Citation:
Assignee:
SHOWA PHARM CHEM IND (JP)
MURATA ATSUSHI (JP)
MURATA ATSUSHI (JP)
International Classes:
C12Q1/34; (IPC1-7): C12Q1/34
Domestic Patent References:
| WO2002024707A1 | 2002-03-28 | |||
| WO1992019763A1 | 1992-11-12 | |||
| WO2002074287A2 | 2002-09-26 | |||
| WO1996021039A1 | 1996-07-11 | |||
| WO1996030540A2 | 1996-10-03 | |||
| WO2002024707A1 | 2002-03-28 |
Foreign References:
| US3830700A | 1974-08-20 | |||
| JP2000224998A | 2000-08-15 | |||
| US5061702A | 1991-10-29 | |||
| JPS4850787A | 1973-07-17 |
Other References:
HURLBUT S. ET AL: "Imipenem Resistance in Bacteroides distasonis Mediated by a Novel b-Lactamase", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 34, no. 1, 1990, pages 117 - 120, XP002977701
P. C. APPELBAUM ET AL: "Characterization of a b-Lactamase from Clostridium clostridioforme", JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, vol. 33, 1994, pages 33 - 40, XP008033659
M. A. CROSBY ET AL: "Activity of Cefoperazone and two b-Lactamase Inhibitors, Sulbactam and Clavulanic Acid, Against Bacteroides spp. correlated with b-Lactamase Production", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 22, no. 3, 1982, pages 398 - 405, XP002977703
P. C. APPELBAUM ET AL: "Characterization of a b-Lactamase from Clostridium clostridioforme", JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, vol. 33, 1994, pages 33 - 40, XP008033659
M. A. CROSBY ET AL: "Activity of Cefoperazone and two b-Lactamase Inhibitors, Sulbactam and Clavulanic Acid, Against Bacteroides spp. correlated with b-Lactamase Production", ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, vol. 22, no. 3, 1982, pages 398 - 405, XP002977703
Attorney, Agent or Firm:
Kumakura, Yoshio (Shin-Tokyo Bldg., 3-1, Marunouchi 3-Chom, Chiyoda-Ku Tokyo, JP)
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