Title:
SCREENING SYSTEM BASED ON EXPRESSION OF ABCG2 HALF TRANSPORTER PROTEIN
Document Type and Number:
WIPO Patent Application WO2002071073
Kind Code:
A3
Abstract:
The overexpression of an ABC half-transporter, ABCG2 (MXR/BCRP) transporter causes multidrug resistance in tumors. The present invention is based on the in vitro expression of the ABCG2 protein in insect cells as an expression system free of other, closely related, functional ABC transporters. In particular, the invention is related to a method for testing drugs for their effect on ABCG2 protein, method for the expression of active ABCG2, isolated ABCG2 homooligomers, preferably homodimers and uses thereof for testing drugs. The invention is further related to cell membrane preparations and cells, preferably insect cells comprising active ABCG2 and reagent kits for testing drugs for their effect on an ABCG2 half transporter protein. The invention is also related to a method for identifying ABCG2 activity in a biological sample, utilizing its substrate/inhibitor specificity.
Inventors:
OEZVEGY CSILLA (HU)
SZAKACS GERGELY (HU)
VARADI ANDRAS (HU)
NAGY ZOLTAN (HU)
SZAKACS GERGELY (HU)
VARADI ANDRAS (HU)
NAGY ZOLTAN (HU)
Application Number:
PCT/HU2002/000015
Publication Date:
April 03, 2003
Filing Date:
March 04, 2002
Export Citation:
Assignee:
SOLVO BIOTECHNOLOGY INC (HU)
OEZVEGY CSILLA (HU)
SZAKACS GERGELY (HU)
VARADI ANDRAS (HU)
NAGY ZOLTAN (HU)
OEZVEGY CSILLA (HU)
SZAKACS GERGELY (HU)
VARADI ANDRAS (HU)
NAGY ZOLTAN (HU)
International Classes:
G01N33/68; (IPC1-7): G01N33/68; C12N5/00; G01N33/574; G01N33/50
Domestic Patent References:
| WO1999040110A1 | 1999-08-12 | |||
| WO2000069390A2 | 2000-11-23 | |||
| WO2002028894A1 | 2002-04-11 |
Other References:
OZVEGY CSILLA ET AL: "Functional characterization of the human multidrug transporter, ABCG2, expressed in insect cells.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 285, no. 1, 6 July 2001 (2001-07-06), pages 111 - 117, XP002219659, ISSN: 0006-291X
ALLEN JOHN D ET AL: "The mouse Bcrp1/Mxr/Abcp gene: Amplification and overexpression in cell lines selected for resistance to topotecan, mitoxantrone, or doxorubicin.", CANCER RESEARCH, vol. 59, no. 17, pages 4237 - 4241, XP002219660, ISSN: 0008-5472
CHEN WENDY S ET AL: "Effects of MDR1 and MDR3 P-glycoproteins, MRP1, and BCRP/MXR/ABCP on the transport of 99mTc-Tetrofosmin.", BIOCHEMICAL PHARMACOLOGY, vol. 60, no. 3, 2000, pages 413 - 426, XP002219661, ISSN: 0006-2952
DOYLE L A ET AL: "A MULTIDRUG RESISTANCE TRANSPORTER FROM HUMAN MCF-7 BREAST CANCER CELLS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 95, December 1998 (1998-12-01), pages 15665 - 15670, XP001055044, ISSN: 0027-8424
ALLEN JOHN D ET AL: "The mouse Bcrp1/Mxr/Abcp gene: Amplification and overexpression in cell lines selected for resistance to topotecan, mitoxantrone, or doxorubicin.", CANCER RESEARCH, vol. 59, no. 17, pages 4237 - 4241, XP002219660, ISSN: 0008-5472
CHEN WENDY S ET AL: "Effects of MDR1 and MDR3 P-glycoproteins, MRP1, and BCRP/MXR/ABCP on the transport of 99mTc-Tetrofosmin.", BIOCHEMICAL PHARMACOLOGY, vol. 60, no. 3, 2000, pages 413 - 426, XP002219661, ISSN: 0006-2952
DOYLE L A ET AL: "A MULTIDRUG RESISTANCE TRANSPORTER FROM HUMAN MCF-7 BREAST CANCER CELLS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 95, December 1998 (1998-12-01), pages 15665 - 15670, XP001055044, ISSN: 0027-8424
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