METHOD FOR ANALYZING PROTEINS

WIPO Patent Application WO2002071074

A3

A method for characterizing an individual protein contained in a complex mixture of proteins includes the steps of: providing a mi xture containing different proteins; fragmenting at least one of the proteins contained in the mixture into a terminal peptide and a non-terminal peptide; separating the terminal peptide from the non-terminal peptide; and analyzing at least one chemical characteristic of the terminal peptide.

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FOOTE ROBERT S
RAMSEY MICHAEL J

PCT/US2002/000369

March 20, 2003

January 09, 2002

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UT BATTELLE LLC (US)

C12Q1/37; G01N33/68; (IPC1-7): G01N33/68; C12Q1/37

WO2000020870A1

2000-04-13

US6156527A

2000-12-05

SECHI S ET AL: "A method to define the carboxyl terminal of proteins.", ANALYTICAL CHEMISTRY. UNITED STATES 15 JUL 2000, vol. 72, no. 14, 15 July 2000 (2000-07-15), pages 3374 - 3378, XP002223787, ISSN: 0003-2700
GENG M ET AL: "Signature-peptide approach to detecting proteins in complex mixtures", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 870, no. 1-2, February 2000 (2000-02-01), pages 295 - 313, XP004187331, ISSN: 0021-9673
WILKINS M R ET AL: "PROTEIN IDENTIFICATION WITH N AND C-TERMINAL SEQUENCE TAGS IN PROTEOME PROJECTS", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 278, 1998, pages 599 - 608, XP002930600, ISSN: 0022-2836

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