Title:
STEM CELL LIBRARIES
Document Type and Number:
WIPO Patent Application WO2004042023
Kind Code:
B1
Abstract:
A stem cell library is created by genetically modifying stem cells with nucleic acids encoding polypeptides which can promote stem cell differentiation into specific cell types. Alternatively, the stem cell library is exposed to an externally added factor that promotes stem cell differentiation into a desired cell line, e.g., neuronal or muscle. The library is used to determine the effect of the encoded protein on the differentiation process. The library is also used to produce nucleic acids for insertion into embryonic stem cells to produce transfected embryonic stem cells. The nucleic acids are inserted into a locus that permits widespread expression of the encoded polypeptide in animals produced from blastocysts that incorporate the transfected cells. Non-human chimeric animals produced by combining blastocysts derived from animal models of human disease and embryonic stem cells transfected with molecules from the library provide an in vivo system for therapeutic design.
Inventors:
ZHANG HONGBING (US)
WILLIAMS LEWIS T (US)
CHU KETTING (US)
COHEN FRED (US)
WILLIAMS LEWIS T (US)
CHU KETTING (US)
COHEN FRED (US)
Application Number:
PCT/US2003/034811
Publication Date:
October 21, 2004
Filing Date:
October 31, 2003
Export Citation:
Assignee:
FIVE PRIME THERAPEUTICS INC (US)
ZHANG HONGBING (US)
WILLIAMS LEWIS T (US)
CHU KETTING (US)
COHEN FRED (US)
ZHANG HONGBING (US)
WILLIAMS LEWIS T (US)
CHU KETTING (US)
COHEN FRED (US)
International Classes:
A01K67/00; A01K67/033; C12N5/00; C12N5/02; C12N5/0735; C12N15/85; G01N33/00; C12N; (IPC1-7): G01N33/00; A01K67/00; A01K67/033; C12N5/00; C12N5/02
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