Title:
SYSTEM AND METHOD FOR SITE-SPECIFIC INTEGRATION OF EXOGENOUS GENES
Document Type and Number:
WIPO Patent Application WO/2023/029492
Kind Code:
A1
Abstract:
A method for site-specific integration of exogenous genes. The method does not rely on the linearization of a homologous arm and a donor vector. The present invention also relates to a system and a kit for editing a nucleic acid and the use thereof, and a method for editing a nucleic acid. The system, the kit and the method can be used to break one nucleic acid strand of a double-stranded target nucleic acid and form a flap protruding at the end (especially the end generated by the break) thereof, and can be used to insert the target nucleic acid into a nucleic acid molecule of interest (e.g. genomic DNA) or replace a nucleotide fragment in the nucleic acid molecule of interest (e.g. genomic DNA) with the target nucleic acid.
Inventors:
LI WEI (CN)
ZHOU QI (CN)
WANG CHENXIN (CN)
FANG SEN (CN)
CHEN YANGCAN (CN)
ZHOU QI (CN)
WANG CHENXIN (CN)
FANG SEN (CN)
CHEN YANGCAN (CN)
Application Number:
PCT/CN2022/086979
Publication Date:
March 09, 2023
Filing Date:
April 15, 2022
Export Citation:
Assignee:
INST ZOOLOGY CAS (CN)
BEIJING INST FOR STEM CELL AND REGENERATIVE MEDICINE (CN)
BEIJING INST FOR STEM CELL AND REGENERATIVE MEDICINE (CN)
International Classes:
C12N9/12; C12N5/10; C12N9/22; C12N15/11; C12N15/113; C12N15/62
Foreign References:
| CN113913405A | 2022-01-11 | |||
| CN112195164A | 2021-01-08 | |||
| CN108690845A | 2018-10-23 | |||
| US20190359973A1 | 2019-11-28 | |||
| CN108070610A | 2018-05-25 |
Other References:
FU YA-WEN, DAI XIN-YUE, WANG WEN-TIAN, YANG ZHI-XUE, ZHAO JUAN-JUAN, ZHANG JIAN-PING, WEN WEI, ZHANG FENG, OBERG KERBY C, ZHANG LE: "Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 49, no. 2, 25 January 2021 (2021-01-25), GB , pages 969 - 985, XP093042374, ISSN: 0305-1048, DOI: 10.1093/nar/gkaa1251
JAYAVARADHAN RAJESWARI, PILLIS DEVIN M., GOODMAN MICHAEL, ZHANG FAN, ZHANG YUE, ANDREASSEN PAUL R., MALIK PUNAM: "CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites", NATURE COMMUNICATIONS, vol. 10, no. 1, 1 December 2019 (2019-12-01), XP055775648, DOI: 10.1038/s41467-019-10735-7
JAYAVARADHAN RAJESWARI, PILLIS DEVIN M., GOODMAN MICHAEL, ZHANG FAN, ZHANG YUE, ANDREASSEN PAUL R., MALIK PUNAM: "CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites", NATURE COMMUNICATIONS, vol. 10, no. 1, 1 December 2019 (2019-12-01), XP055775648, DOI: 10.1038/s41467-019-10735-7
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (CN)
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