TRANSGENIC YEAST, AND METHOD FOR PRODUCTION OF ETHANOL

WIPO Patent Application WO/2010/005044

A1

Disclosed is transgenic yeast which has an excellent xylose metabolism rate even in the presence of a co-existing sugar.  Also disclosed is a method for producing ethanol by utilizing the transgenic yeast. The transgenic yeast has two or more copies of a cell-surface-presenting β-glucosidase gene and two or more copies of a xylose-metabolism-related gene introduced into the genome thereof or has a cell-surface-presenting β-glucosidase gene and two or more copies of a xylose-metabolism-related gene introduced into the genome thereof, and has a β-glucosidase activity (i.e., a 0.02 U/OD660 value) of 1 or greater.

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SAITOH SATOSHI (JP)
TAKAHASHI KAZUSHI (JP)
MIYATA KAYO (JP)
SUZUKI EMIKO (JP)
KONDO AKIHIKO (JP)

PCT/JP2009/062502

January 14, 2010

July 09, 2009

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TOYOTA MOTOR CO LTD (JP)
NAT UNIV CORP KOBE UNIV (JP)
SAITOH SATOSHI (JP)
TAKAHASHI KAZUSHI (JP)
MIYATA KAYO (JP)
SUZUKI EMIKO (JP)
KONDO AKIHIKO (JP)

C12N1/19; C12N15/09; C12P7/06

KATAHIRA, SATOSHI ET AL.: "Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose- and cellooligosaccharide- assimilating yeast strain.", APPL. MICROBIOL. BIOTECHNOL., vol. 72, 2006, pages 1136 - 1143
"Dai 60 Kai Abstracts of the Annual Meeting of the Society for Biotechnology, Japan, 11 July, 2008 (11.07.08)", article SATOSHI SAITO ET AL.: "Wine Kobo OC-2 Triple Marker-kabu o Platform to shita Ko-BGL Kassei Kobo to Ko-xylose Taisha Noryoku Kobo no Sakusei", pages: 97
HUGHES, TIMOTHY R. ET AL.: "Functional discovery via a compendium of expression profiles.", CELL, vol. 102, 2000, pages 109 - 126
SAITOH, SATHOSHI ET AL.: "Breeding of Industrial Diploid Yeast Strain with Chromosomal Integration of Multiple beta-Glucosidase Genes.", J. BIOSCI. BIOENG., vol. 106, no. 6, December 2008 (2008-12-01), pages 594 - 597

HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)

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