Title:
超臨界流体クロマトグラフを用いた成分分離方法
Document Type and Number:
Japanese Patent JP6819800
Kind Code:
B2
Abstract:
For the separation of a peptide or protein in a sample using a supercritical fluid chromatograph, carbon dioxide is used as the supercritical fluid, while an organic solvent containing hexafluoroisopropanol is used as the modifier to be added to the supercritical fluid. If a mixed liquid of methanol, isopropanol, water and formic acid is simply used as the modifier, a considerable amount of peak tailing occurs on the chromatogram, causing the peak waveform to be distorted. By comparison, when a mixed liquid prepared by additionally mixing hexafluoroisopropanol in the mixed liquid of methanol, isopropanol, water and formic acid is used, the peak observed on the chromatogram will have a sharp, satisfactory shape.
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Inventors:
Yana Rikuru
Application Number:
JP2019569983A
Publication Date:
January 27, 2021
Filing Date:
November 06, 2017
Export Citation:
Assignee:
SHIMADZU CORPORATION
International Classes:
G01N30/02; G01N30/26; G01N30/88
Domestic Patent References:
| JP2013250186A | ||||
| JP2015194363A | ||||
| JP2015175747A |
Other References:
TAKEUCHI, Makoto et al.,Applications of semi-micro supercritical fluid chromatography with gradient elution to synthetic oligomer separation,Journal of Chromatography,1990年,Vol.515,pp.629-638
Attorney, Agent or Firm:
Kyoto International Patent Office