ADENO-ASSOCIATED VIRUS PURIFICATION METHOD

Title:

ADENO-ASSOCIATED VIRUS PURIFICATION METHOD

Document Type and Number:

Japanese Patent JP2023184470

Kind Code:

A

Abstract:

To provide a method that can purify adeno-associated virus (AAV) in a sample under mild conditions, using affinity chromatography.SOLUTION: A method for purifying adeno-associated virus (AAV) in a sample includes the steps for: adding a sample including AAV to an adsorbent including an insoluble carrier and an AAV-binding protein immobilized to the carrier, causing the AAV to be adsorbed onto the adsorbent; and allowing the AAV adsorbed onto the adsorbent to be eluted using a neutral buffer including 800 mmol/L or more of chloride ions or anions lower in a Hofmeister series than the chloride ions.SELECTED DRAWING: Figure 1

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Inventors:

MAKINO YURIKO
YOSHIDA KOHEI
TANAKA TORU

Application Number:

JP2023095990A

Publication Date:

December 28, 2023

Filing Date:

June 12, 2023

Export Citation:

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Assignee:

TOSOH CORP

International Classes:

C12N15/864; C12N15/13

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