To measure modified hemoglobin even in low concn. with high sensitivity by allowing a water-soluble compd. among polyethylene glycol, polyvinyl pyrrolidone, pullulan and dextrane to be present in a hemolytic sample.
When modified hemoglobin is measured by immunoagglutination reaction, at least one water-soluble compd. selected from the group consisting of polyethylene glycol, polyvinyl pyrrolidone (weight mean mol.wt.; about 3,000-1,000,000), pullulan and dextrane (weight mean mol.wt.; 1,000-1,000,000) is allowed to be present in a hemolytic sample to be used. If a mol.wt. is too small, the enhancement of sensitivity is insufficient and, if too large, solubility becomes bad. The concn. of the water-soluble compd. is set to about 0.01-10% (w/v) of a reaction system. If the concn. of the water-soluble compd. is too low, sensitivity is lowered and, if too high, non-specific reaction is generated and accurate measurement becomes difficult. By this method, the sensitivity of immunoagglutination reaction can be enhanced drastically.
IWAMOTO HISAHIKO
YOSHIMURA YOSHINORI
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