Title:
METHOD FOR DETECTING DNA ENDONUCLEASE ACTIVITY
Document Type and Number:
Japanese Patent JP2003088369
Kind Code:
A
Abstract:
To provide a method for detecting DNA endonuclease activity, especially for detecting apoptosis.
Disclosed is a method for detecting the DNA endonuclease activity featured by comprising (a) a process preparing a substrate DNA labeled by a fluorescent marker, (b) a process performing digestion reaction of the substrate DNA by mixing a sample to measure the DNA endonuclease activity with the substrate DNA, (c) a process measuring fluorescence intensity from the fluorescent marker of the substrate DNA at plural elapsed times in a small space for measurement as performed the digestion reaction, and (d) a process detecting the decomposition of the substrate DNA based on flactuation with the lapse of time of the measured fluorescence intensity.
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Inventors:
TAKAHASHI TAKEO
Application Number:
JP2001244783A
Publication Date:
March 25, 2003
Filing Date:
August 10, 2001
Export Citation:
Assignee:
OLYMPUS OPTICAL CO
International Classes:
C12N15/09; C12Q1/42; (IPC1-7): C12N15/09; C12Q1/42
Domestic Patent References:
| JP2000125900A | 2000-05-09 | |||
| JPH08187079A | 1996-07-23 | |||
| JP2000166598A | 2000-06-20 |
Attorney, Agent or Firm:
Takehiko Suzue (4 outside)