To provide a new method for producing D-fructose differing in fundamental principle from the conventional glucose isomerization method, comprising oxidative fermentation of D-sorbitol into D-fructose in nearly 100% yield using aerobic bacteria, especially acetic acid bacteria, through predicting the possibility of the oxidative fermentation using an enzyme not in accordance with the Bertrand-Hudson rule based on a study for the fundamental analysis of oxidative fermentation for many years.
This method for producing D-fructose from D-sorbitol involves the oxidative fermentation utilizing FAD-SLDH (a specific sorbitol dehydrogenase). This new method is greatly superior to the conventional glucose isomerization method having many shortcomings in terms of D-fructose yield, production efficiency and production cost.
MATSUSHITA KAZUNOBU
TOYAMA HIROHIDE
SHINAGAWA EMIKO
GANJANA TEERAGUURU
JUANGUTEIPPU MUUNMANMII
KASETSART UNIV
KING MONGKUT S UNIV OF TECHNOL
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