Title:
COMPOSITIONS AND METHODS FOR AFFECTING VIRULENCE DETERMINANTS IN BACTERIA
Document Type and Number:
WIPO Patent Application WO2002068610
Kind Code:
A3
Abstract:
A novel sar R gene and sar R gene product which down regulates the expression of sar A and the resulting virulence determination in Staphylococcus aureus is provided. Methods for modulating the expression of sar A and virulence determinants are also provided. A preferred embodiment of the present invention provides structural information relating to the gene product and enables the identification and formulation of lead compounds and reducements for treating and preventing infections by S. aureus and related bacteria.
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Inventors:
CHEUNG AMBROSE L
MANNA ADHAR
ZHANG GONGYI
MANNA ADHAR
ZHANG GONGYI
Application Number:
PCT/US2002/000877
Publication Date:
October 30, 2003
Filing Date:
January 11, 2002
Export Citation:
Assignee:
DARTMOUTH COLLEGE (US)
International Classes:
C07K14/31; A61K38/00; (IPC1-7): C12Q1/68; C07H21/02; C12N15/00; G01N33/53
Foreign References:
| US5976792A | 1999-11-02 | |||
| US5587288A | 1996-12-24 |
Other References:
CHEUNG ET AL.: "Regulation of exoprotein expression by staphylococcus aureus by a locus (sar) distinct from Agr", PROC. NATL. ACAD. SCI. USA, vol. 89, July 1992 (1992-07-01), pages 6462 - 6466, XP000670077
CHEUNG ET AL.: "Cloning and sequencing of sarA of staphylococcus aureus, a gene required for the expression of agr", J. BACTERIOLOGY, vol. 176, no. 13, July 1994 (1994-07-01), pages 4168 - 4172, XP000645499
MANNA ET AL.: "Transcriptional analysis of different promoters in the sar locus in staphylococcus aureus", J. BACTERIOLOGY, vol. 180, no. 15, August 1998 (1998-08-01), pages 3828 - 3836, XP002965964
DATABASE GENBANK [online] NATIONAL CENTER FOR BIOTECHNOLOGY; 22 November 1999 (1999-11-22), "Direct submission, gene sequence", XP002965965, accession no. NCBI Database accession no. (AF207701)
DATABASE GENBANK [online] SIB(SWISS) INSTITUTE FOR BIOINFORMATICS, (GENEVA SWITZERLAND); 22 November 1999 (1999-11-22), "Characterization of SarR, a modulator of sar expression in staphylococcus aureus", XP002965966, accession no. NCBI Database accession no. (Q9F0R1)
MANNA ET AL.: "Characterization of sarR, a modulator of sar expression in staphylococcus aureus", INFECT. IMMUN., vol. 69, no. 2, February 2001 (2001-02-01), pages 885 - 896, XP002965967
CHEUNG ET AL.: "Global regulation of virulence determinants in staphylococcus aureus by the SarA protein family", FRONTIERS IN BIOSCIENCE, vol. 7, pages 1825 - 1842, XP002965968
CHEUNG ET AL.: "Role of the sar locus of staphylococcus aureus in induction of endocarditis in rabbits", INFECT. IMMUN., vol. 62, no. 5, May 1994 (1994-05-01), pages 1719 - 1725, XP002966127
CHEUNG ET AL.: "Cloning and sequencing of sarA of staphylococcus aureus, a gene required for the expression of agr", J. BACTERIOLOGY, vol. 176, no. 13, July 1994 (1994-07-01), pages 4168 - 4172, XP000645499
MANNA ET AL.: "Transcriptional analysis of different promoters in the sar locus in staphylococcus aureus", J. BACTERIOLOGY, vol. 180, no. 15, August 1998 (1998-08-01), pages 3828 - 3836, XP002965964
DATABASE GENBANK [online] NATIONAL CENTER FOR BIOTECHNOLOGY; 22 November 1999 (1999-11-22), "Direct submission, gene sequence", XP002965965, accession no. NCBI Database accession no. (AF207701)
DATABASE GENBANK [online] SIB(SWISS) INSTITUTE FOR BIOINFORMATICS, (GENEVA SWITZERLAND); 22 November 1999 (1999-11-22), "Characterization of SarR, a modulator of sar expression in staphylococcus aureus", XP002965966, accession no. NCBI Database accession no. (Q9F0R1)
MANNA ET AL.: "Characterization of sarR, a modulator of sar expression in staphylococcus aureus", INFECT. IMMUN., vol. 69, no. 2, February 2001 (2001-02-01), pages 885 - 896, XP002965967
CHEUNG ET AL.: "Global regulation of virulence determinants in staphylococcus aureus by the SarA protein family", FRONTIERS IN BIOSCIENCE, vol. 7, pages 1825 - 1842, XP002965968
CHEUNG ET AL.: "Role of the sar locus of staphylococcus aureus in induction of endocarditis in rabbits", INFECT. IMMUN., vol. 62, no. 5, May 1994 (1994-05-01), pages 1719 - 1725, XP002966127
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