Title:
METHOD FOR FORMING SHEET OF CARDIOMYOCYTES
Document Type and Number:
WIPO Patent Application WO/2021/065984
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing: a method for producing a high-quality sheet-like cell culture containing a variety of cells such as cardiomyocytes, in particular, cardiomyocytes derived from pluripotent stem cells, the cells highly maintaining the function thereof; a sheet-like cell culture produced using the method; and a method for treating a disease using the sheet-like cell culture. The problem is solved by a sheet-like cell culture production method comprising: (a) a step of plating a cell population including cardiomyocytes on a culture substrate; and (b) a step of culturing the plated cell population with a sheet formation medium including a cell adhesive component to form a sheet.
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Inventors:
SAWA YOSHIKI (JP)
MIYAGAWA SHIGERU (JP)
OYAMA KENJI (JP)
OHASHI FUMIYA (JP)
MIYAGAWA SHIGERU (JP)
OYAMA KENJI (JP)
OHASHI FUMIYA (JP)
Application Number:
PCT/JP2020/037084
Publication Date:
April 08, 2021
Filing Date:
September 30, 2020
Export Citation:
Assignee:
UNIV OSAKA (JP)
TERUMO CORP (JP)
TERUMO CORP (JP)
International Classes:
A61P9/00; A61K35/34; A61L27/22; A61L27/36; A61L27/38; A61L27/40; C12N5/07; C12Q1/02
Domestic Patent References:
| WO2016076368A1 | 2016-05-19 |
Other References:
BURNOUF, T. ET AL.: "Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation", BIOMATERIALS, vol. 76, 2016, pages 371 - 387, XP029317322, DOI: 10.1016/j.biomaterials.2015.10.065
SAPORITO, FRANCESCA, SANDRI GIUSEPPINA; BONFERONI MARIA CRISTINA; ROSSI SILVIA; MALAVASI LORENZO; FANTE CLAUDIA DEL; VIGANI BARBAR: "Electrospun gelatin-chondroitin sulfate scaffolds loaded with platelet lysate promote immature cardiomyocyte proliferation", POLYMERS, vol. 10, no. 208, 21 February 2018 (2018-02-21), pages 1 - 20, XP055701574, DOI: 10.3390/polym10020208
SAPORITO, FRANCESCA, SANDRI GIUSEPPINA; BONFERONI MARIA CRISTINA; ROSSI SILVIA; MALAVASI LORENZO; FANTE CLAUDIA DEL; VIGANI BARBAR: "Electrospun gelatin-chondroitin sulfate scaffolds loaded with platelet lysate promote immature cardiomyocyte proliferation", POLYMERS, vol. 10, no. 208, 21 February 2018 (2018-02-21), pages 1 - 20, XP055701574, DOI: 10.3390/polym10020208
Attorney, Agent or Firm:
KUZUWA, Kiyoshi (JP)
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