METHOD FOR IDENTIFICATION OF VARIETY OF HEVEA BRASILIENSIS, AND KIT FOR IDENTIFICATION OF VARIETY OF HEVEA BRASILIENSIS

WIPO Patent Application WO/2009/091074

A1

Disclosed is a method which can identify the variety of a plant Hevea brasiliensis accurately and in a simple manner. Specifically disclosed is a method for identifying the variety of a plant Hevea brasiliensis, which is characterized by identifying the variety of the plant Hevea brasiliensis based on at least one criterion selected from the following items: the presence or absence of a DNA fragment which is amplified by carrying out PCR by using genomic DNA extracted from the plant Hevea brasiliensis as a template and using at least one primer set selected from the following primer sets: a primer comprising the nucleotide sequence depicted in SEQ ID NO:1 and a primer comprising the nucleotide sequence depicted in SEQ ID NO:2; a primer comprising the nucleotide sequence depicted in SEQ ID NO:3 and a primer comprising the nucleotide sequence depicted in SEQ ID NO:4; a primer comprising the nucleotide sequence depicted in SEQ ID NO:5 and a primer comprising the nucleotide sequence depicted in SEQ ID NO:6; a primer comprising the nucleotide sequence depicted in SEQ ID NO:7 and a primer comprising the nucleotide sequence depicted in SEQ ID NO:8; a primer comprising the nucleotide sequence depicted in SEQ ID NO:9 and a primer comprising the nucleotide sequence depicted in SEQ ID NO:10; a primer comprising the nucleotide sequence depicted in SEQ ID NO:11 and a primer comprising the nucleotide sequence depicted in SEQ ID NO:12; a primer comprising the nucleotide sequence depicted in SEQ ID NO:13 and a primer comprising the nucleotide sequence depicted in SEQ ID NO:14; and a primer comprising the nucleotide sequence depicted in SEQ ID NO:15 and a primer comprising the nucleotide sequence depicted in SEQ ID NO:16; the length of the DNA fragment; and the digestion pattern of the DNA fragment with a restriction enzyme. Also disclosed is a variety identification kit for achieving the method.

WO/2002/053719	CYTOSKELETON-ASSOCIATED PROTEINS
WO/2002/063001	ISOLATED HUMAN G-PROTEIN COUPLED RECEPTORS, NUCLEIC ACID MOLECULES ENCODING HUMAN GPCR PROTEINS, AND USES THEREOF
WO/1996/026270	DNA MOLECULES, PREPARATION THEREOF AND USE THEREOF IN GENE THERAPY

OKUMURA SATORU (JP)

PCT/JP2009/050914

July 23, 2009

January 15, 2009

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BRIDGESTONE CORP (JP)
BADAN PENGKAJIAN DAN PENERAPAN (ID)
OKUMURA SATORU (JP)

C12Q1/68; C12N15/09

DATABASE GENBANK DDBJ/EMBL; 14 April 2004 (2004-04-14), SEGUIN, M. ET AL., retrieved from http://www.ncbi.nlm.nih.gov/ entrez/viewer.fcgi?db=nuccore&id=46326480 Database accession no. AY486624
DATABASE GENBANK DDBJ/EMBL; 21 April 2005 (2005-04-21), SAHA, T. ET AL., retrieved from http://www.ncbi.nlm.nih.gov/ entrez/viewer.fcgi?db=nuccore&id=23296058 Database accession no. AY135656
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PARAN, I. ET AL.: "Development of reliable PCR- based markers linked to downy mildew resistance genes in lettuce.", THEORETICAL AND APPLIED GENETICS, vol. 85, 1993, pages 985 - 993
LEE, G.P. ET AL.: "Molecular markers derived from RAPD, SCAR, and the conserved 18S rDNA sequences for classification and identification in Pyrus pyrifolia and P. communis.", THEORETICAL AND APPLIED GENETICS, vol. 108, 2004, pages 1487 - 1491

SUGIMURA, Kenji et al. (3-2-1 Kasumigaseki,Chiyoda-k, Tokyo 13, JP)

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