Title:
POLYPHOSPHATE KINASE RMPPK, AND CODING GENE AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/031170
Kind Code:
A1
Abstract:
A polyphosphate kinase RmPPK, and a coding gene and an application thereof. The amino acid sequence of the polyphosphate kinase RmPPK is: the amino acid sequence shown in SEQ ID NO. 1; or an amino acid sequence in which the amino acid sequence shown in SEQ ID NO. 1 is modified by substituting, deleting, or adding one or more amino acids and having the same functions; or an amino acid sequence over 60% homologous with the amino acid sequence shown in SEQ ID NO.1 and having the same functions. A polyphosphate kinase gene RmPPK is introduced into E. coli BL21 (DE3) for high-efficiency expression in order to acquire the polyphosphate kinase RmPPK. A tool enzyme for the regeneration of CTP, CMP-sialic acid, UTP, UDP-Glc/UDP-GlcA/UDP-GlcNAc/UDP-Gal/UDP-GalNAc/UDP-Xyl, GTP or GDP-Fuc/GDP-Man is thus provided.
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Inventors:
LI JIANJUN (CN)
DU YUGUANG (CN)
XU HUA (CN)
SUN MING (CN)
WANG ZHUO (CN)
JIAO SIMING (CN)
REN LISHI (CN)
DU YUGUANG (CN)
XU HUA (CN)
SUN MING (CN)
WANG ZHUO (CN)
JIAO SIMING (CN)
REN LISHI (CN)
Application Number:
PCT/CN2019/101864
Publication Date:
February 25, 2021
Filing Date:
August 21, 2019
Export Citation:
Assignee:
ZHONGKE RUNXIN SUZHOU BIOLOGICAL TECH CO LTD (CN)
International Classes:
C12N9/12; C12N15/54; C12P19/32
Foreign References:
| CN108795958A | 2018-11-13 | |||
| CN105463043A | 2016-04-06 | |||
| CN109161536A | 2019-01-08 | |||
| CN109385390A | 2019-02-26 |
Other References:
DATABASE PROTEIN 20-06-2019, ANONYMOUS: "polyphosphate kinase 2 [Ruegeria marina]", XP055783035, retrieved from GENBANK Database accession no. WP_093026974
K. ISHIGE, H. ZHANG, A. KORNBERG: "Polyphosphate kinase (PPK2), a potent, polyphosphate-driven generator of GTP", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 99, no. 26, 24 December 2002 (2002-12-24), US, pages 16684 - 16688, XP055377974, ISSN: 0027-8424, DOI: 10.1073/pnas.262655299
ISHIGE K. ET AL.: "Novel method for enzymatic synthesis of CMP-NeuAc", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, vol. 65, no. 8, 1 August 2001 (2001-08-01), pages 1736 - 1740, XP002983840
WANG JUNZHI; ZHENG CHENG; ZHANG TIANYI; LIU YINGMIAO; CHENG ZHUOPEI; LIU DONG; YING HANJIE; NIU HUANQING: "Novel one-pot ATP regeneration system based on three-enzyme cascade for industrial CTP production", BIOTECHNOLOGY LETTERS, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, vol. 39, no. 12, 31 August 2017 (2017-08-31), Dordrecht, pages 1875 - 1881, XP036355027, ISSN: 0141-5492, DOI: 10.1007/s10529-017-2427-x
K. ISHIGE, H. ZHANG, A. KORNBERG: "Polyphosphate kinase (PPK2), a potent, polyphosphate-driven generator of GTP", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 99, no. 26, 24 December 2002 (2002-12-24), US, pages 16684 - 16688, XP055377974, ISSN: 0027-8424, DOI: 10.1073/pnas.262655299
ISHIGE K. ET AL.: "Novel method for enzymatic synthesis of CMP-NeuAc", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, vol. 65, no. 8, 1 August 2001 (2001-08-01), pages 1736 - 1740, XP002983840
WANG JUNZHI; ZHENG CHENG; ZHANG TIANYI; LIU YINGMIAO; CHENG ZHUOPEI; LIU DONG; YING HANJIE; NIU HUANQING: "Novel one-pot ATP regeneration system based on three-enzyme cascade for industrial CTP production", BIOTECHNOLOGY LETTERS, KLUWER ACADEMIC PUBLISHERS, DORDRECHT, vol. 39, no. 12, 31 August 2017 (2017-08-31), Dordrecht, pages 1875 - 1881, XP036355027, ISSN: 0141-5492, DOI: 10.1007/s10529-017-2427-x
Attorney, Agent or Firm:
SHANGHAI SHUONEE INTELLECTUAL PROPERTY LAW OFFICE (CN)
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