Title:
COMPOSITIONS AND METHODS FOR PLANT GENETIC MODIFICATION
Document Type and Number:
WIPO Patent Application WO2001025460
Kind Code:
A3
Abstract:
Mixed duplex oligonucleotides (MDON) are used to effect site-specific genetic alterations in a target DNA sequence of a plant. The MDON are introduced by electroporation into microspores. Thereafter, plants having a desired genetic alteration are produced by germinating the microspores.
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Inventors:
BEETHAM PETER
AVISSAR PATRICIA
WALKER KEITH
AVISSAR PATRICIA
WALKER KEITH
Application Number:
PCT/US2000/027870
Publication Date:
March 07, 2002
Filing Date:
October 06, 2000
Export Citation:
Assignee:
VALIGEN INC (US)
International Classes:
C12N15/10; C12N15/82; (IPC1-7): C12N15/82; C12N15/10; A01H5/00
Domestic Patent References:
| WO1999007865A1 | 1999-02-18 | |||
| WO1993021335A2 | 1993-10-28 | |||
| WO1997048714A1 | 1997-12-24 | |||
| WO1995015972A1 | 1995-06-15 |
Other References:
SOUVRE A ET AL: "Transformation of rape (Brassica napus L.) through the haploid embryogenesis pathway.", ACTA SOCIETATIS BOTANICORUM POLONIAE, vol. 65, no. 1-2, 1996, Seventh Conference of Plant Embryologists of Czech Republic, Slovakia and Poland;Lublin, Poland; September 14-16, 1995, pages 194 - 195, XP001002611, ISSN: 0001-6977
FUKUOKA H ET AL: "Direct gene delivery into isolated microspores of rapeseed (Brassica napus L.) and the production of fertile transgenic plants.", PLANT CELL REPORTS, vol. 17, no. 5, March 1998 (1998-03-01), pages 323 - 328, XP002170036, ISSN: 0721-7714
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, YAO QING A ET AL: "Biolistic transformation of haploid isolated microspores of barley (Hordeum vulgare L.).", XP002170037, Database accession no. PREV199799738285
JARDINAUD MARIE-FRANCOISE ET AL: "Transient GUS gene expression in Brassica napus electroporated microspores.", PLANT SCIENCE (LIMERICK), vol. 93, no. 1-2, 1993, pages 177 - 184, XP001002561, ISSN: 0168-9452
FUKUOKA H ET AL: "Direct gene delivery into isolated microspores of rapeseed (Brassica napus L.) and the production of fertile transgenic plants.", PLANT CELL REPORTS, vol. 17, no. 5, March 1998 (1998-03-01), pages 323 - 328, XP002170036, ISSN: 0721-7714
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, YAO QING A ET AL: "Biolistic transformation of haploid isolated microspores of barley (Hordeum vulgare L.).", XP002170037, Database accession no. PREV199799738285
JARDINAUD MARIE-FRANCOISE ET AL: "Transient GUS gene expression in Brassica napus electroporated microspores.", PLANT SCIENCE (LIMERICK), vol. 93, no. 1-2, 1993, pages 177 - 184, XP001002561, ISSN: 0168-9452
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