Title:
COMPOSITION FOR CLEAVING TARGET DNA COMPRISING GUIDE RNA SPECIFIC FOR TARGET DNA AND CAS PROTEIN-ENCODING NUCLEIC ACID OR CAS PROTEIN, AND USE THEREOF
Document Type and Number:
Japanese Patent JP2023166400
Kind Code:
A
Abstract:
To provide a composition and a method for inducing the modification of a target endogenous nucleic acid sequence in the nucleus of a eukaryotic cell.SOLUTION: The present invention provides a composition for inducing the modification of a target endogenous nucleic acid sequence in the nucleus of a eukaryotic cell, including a Cas9/RNA complex. The complex includes recombinant Cas9 protein, and a guide RNA including crRNA and tracrRNA. The Cas9/RNA complex is a combination of the recombinant Cas9 protein and the guide RNA. The combination is assembled through the in vitro mixing and incubation of the recombinant Cas9 protein with the guide RNA. The guide RNA is either transcribed in vitro or synthesized chemically. The target endogenous nucleic acid sequence includes a part complementary to the crRNA of the guide RNA. The combination of the recombinant Cas9 protein and the guide RNA is introduced into a eukaryotic cell, causing modification of the target endogenous nucleic acid sequence in the nucleus of the eukaryotic cell.SELECTED DRAWING: Figure 1a
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Inventors:
KIM JIN-SOO
CHO SEUNG WOO
KIM SO JUNG
KIM JONG MIN
KIM SEOKJOONG
CHO SEUNG WOO
KIM SO JUNG
KIM JONG MIN
KIM SEOKJOONG
Application Number:
JP2023131349A
Publication Date:
November 21, 2023
Filing Date:
August 10, 2023
Export Citation:
Assignee:
TOOLGEN INC
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N5/10; C12N9/10; C12N15/11
Attorney, Agent or Firm:
Shinichiro Tanaka
▲吉▼田 和彦
Hiroyuki Suda
Kazuo Yamazaki
Satsuki Ichikawa
Hironobu Hattori
Nanae Matsuda
Gen Tashiro
▲吉▼田 和彦
Hiroyuki Suda
Kazuo Yamazaki
Satsuki Ichikawa
Hironobu Hattori
Nanae Matsuda
Gen Tashiro